Overview

  • Product nameAnti-ME3 antibody
    See all ME3 primary antibodies
  • Description
    Rabbit polyclonal to ME3
  • SpecificityThis antibody detects endogenous levels of total ME3 protein.
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    A synthesized peptide derived from an internal region of human ME3.

  • Positive control
    • Extracts from 3T3 cells and RAW264.7 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab74258 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 67 kDa.
ELISA 1/40000.

Target

  • RelevanceMalic enzyme catalyzes the oxidative decarboxylation of malate to pyruvate using either NAD+ or NADP+ as a cofactor. Mammalian tissues contain 3 distinct isoforms of malic enzyme: a cytosolic NADP(+)-dependent isoform, a mitochondrial NADP(+) dependent isoform, and a mitochondrial NAD(+) dependent isoform. This gene encodes a mitochondrial NADP(+) dependent isoform. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined. ME3 is expressed predominantly in organs with a low division rate. Its catalytic activity is (S) malate + NADP+ = pyruvate + CO2 + NADPH.
  • Cellular localizationMitochondrion matrix.
  • Database links
  • Alternative names
    • Malate dehydrogenase antibody
    • Malic enzyme 3 antibody
    • Malic enzyme 3, NADP(+) dependent, mitochondrial antibody
    • NADP ME antibody
    • NADPME antibody
    • Pyruvic malic carboxylase antibody
    see all

Anti-ME3 antibody images

  • All lanes : Anti-ME3 antibody (ab74258) at 1/500 dilution

    Lane 1 : Extracts from 3T3 cells
    Lane 2 : Extracts from RAW264.7 cells
    Lane 3 : Extracts from RAW264.7 cells plus 5µg immunizing peptide

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 67 kDa
    Observed band size : 67 kDa
    Additional bands at : 42 kDa. We are unsure as to the identity of these extra bands.

References for Anti-ME3 antibody (ab74258)

ab74258 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"