Overview

  • Product name
  • Description
    Rabbit polyclonal to MEA1
  • Tested applications
    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence: ALNNHSSIPM DPEHVELVKR TMAGVSLPAP GVPAWAREIS DAQWEDVVQK ALQARQAS, corresponding to internal sequence amino acids 124-181 of Human MEA1.

  • Positive control
    • Human testis tissue; MEA1 over-expression lysate from HEK293 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab121543 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.

Target

Images

  • Immunofluorescent staining of Human cell line A-431 shows positivity in cytoplasm. Recommended concentration of ab121543 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab121543 at 1/20 dilution staining MEA1 in paraffin-embedded Human testis tissue by Immunohistochemistry
  • All lanes : Anti-MEA1 antibody (ab121543) at 1/250 dilution

    Lane 1 : Negative control (vector only transfected HEK293T lysate)
    Lane 2 : MEA1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells

References

ab121543 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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