Anti-Measles nucleoprotein antibody [2D7] (ab9882)

Overview

  • Product nameAnti-Measles nucleoprotein antibody [2D7]
    See all Measles nucleoprotein primary antibodies
  • Description
    Mouse monoclonal [2D7] to Measles nucleoprotein
  • SpecificityReacts with measles NP. Does not cross-react with mumps NP. Recognises both native and recombinant yeast-derived measles NP.
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Immunogen

    Recombinant measles NP

  • Positive control
    • recombinant measles NP

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPBS with 0.1% sodium azide
  • Concentration information loading...
  • PurityIgG fraction
  • ClonalityMonoclonal
  • Clone number2D7
  • MyelomaNS0/1
  • IsotypeIgG1
  • Light chain typeunknown
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab9882 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1000 - 1/10000.
WB 1/500 - 1/2000. Detects a band of approximately 52.5 kDa.

Target

  • RelevanceInvolved in the encapsidating of the genome in a ratio of 1 N per 6 ribonucleotides, resulting in the protection from nucleases. The nucleocapsid (NC) has an helical structure with either 12.35 or 11.64 N per turn, approximately 20nm in diameter, with a hollow central cavity approximately 5nm in diameter. The encapsidated genomic RNA is termed the NC and serves as template for transcription and replication. During replication, encapsidation by N is coupled to RNA synthesis and all replicative products are resistant to nucleases. N is released in the blood following lysis of measles infected cells, it interacts then with human FCGR2B on immune cells, inducing apoptosis and blocking inflammatory immune response. Ntail binds to a protein on human thymic epithelial cells, termed Nucleoprotein Receptor (NR), inducing growth arrest
  • Cellular localizationVirion. Host cytoplasm.
  • Alternative names
    • Measles nucleocapsid protein antibody
    • N antibody
    • NP antibody
    • Nucleocapsid protein antibody

Anti-Measles nucleoprotein antibody [2D7] images

  • Western blot of measles NP using monoclonal antibody 2D7 at a concentration of 0.5 µg/ml.
    Lane 1 - prestained marker proteins
    Lane 2 - recombinant measles NP.

References for Anti-Measles nucleoprotein antibody [2D7] (ab9882)

ab9882 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your enquiry. The molecular weight of the protein (measles nucleocapsid protein) is 52.5 kDa. The size of the orange marker protein is 70 kDa. Please contact us again if you have additional questions.

Thank you for your enquiry about our Measles nucleoprotein antibodies. A nucleoprotein is defined as any of a group of complexes composed of protein and nucleic acid and found in the nuclei and cytoplasm of all living cells, as in chromatin and rib...

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Thank you for the information that you have provided and I'm sorry to hear that your customer is experiencing difficulty with ab9882. We don't have lot 200502 in stock anymore. This is the first complaint that we have received concerning this antibody ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"