Anti-Measles nucleoprotein antibody [3E1] (ab9397)

Overview

  • Product nameAnti-Measles nucleoprotein antibody [3E1]
    See all Measles nucleoprotein primary antibodies
  • Description
    Mouse monoclonal [3E1] to Measles nucleoprotein
  • SpecificityReacts with measles NP. Does not cross-react with mumps NP. Recognises both native and recombinant yeast-derived measles NP.
  • Tested applicationsSuitable for: WB, Indirect ELISAmore details
  • Immunogen

    Recombinant full length protein.

  • Positive control
    • recombinant measles NP

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPBS with 0.1% sodium azide
  • Concentration information loading...
  • PurityIgG fraction
  • ClonalityMonoclonal
  • Clone number3E1
  • MyelomaNS0/1
  • IsotypeIgG1
  • Light chain typeunknown
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab9397 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000.
Indirect ELISA 1/1000 - 1/10000.

Target

  • RelevanceInvolved in the encapsidating of the genome in a ratio of 1 N per 6 ribonucleotides, resulting in the protection from nucleases. The nucleocapsid (NC) has an helical structure with either 12.35 or 11.64 N per turn, approximately 20nm in diameter, with a hollow central cavity approximately 5nm in diameter. The encapsidated genomic RNA is termed the NC and serves as template for transcription and replication. During replication, encapsidation by N is coupled to RNA synthesis and all replicative products are resistant to nucleases. N is released in the blood following lysis of measles infected cells, it interacts then with human FCGR2B on immune cells, inducing apoptosis and blocking inflammatory immune response. Ntail binds to a protein on human thymic epithelial cells, termed Nucleoprotein Receptor (NR), inducing growth arrest
  • Cellular localizationVirion. Host cytoplasm.
  • Alternative names
    • Measles nucleocapsid protein antibody
    • N antibody
    • NP antibody
    • Nucleocapsid protein antibody

Anti-Measles nucleoprotein antibody [3E1] images

  • Immunoblot of measles NP using monoclonal antibody 3E1. Antibody 3E1 at a concentration of 0.5 µg/ml. Lane 1 - prestained marker proteins lane 2 - recombinant measles NP.

References for Anti-Measles nucleoprotein antibody [3E1] (ab9397)

This product has been referenced in:
  • Ito M  et al. Measles virus nonstructural C protein modulates viral RNA polymerase activity by interacting with host protein SHCBP1. J Virol 87:9633-42 (2013). Read more (PubMed: 23804634) »

See 1 Publication for this product

Product Wall

Thank you for your enquiry about our Measles nucleoprotein antibodies. A nucleoprotein is defined as any of a group of complexes composed of protein and nucleic acid and found in the nuclei and cytoplasm of all living cells, as in chromatin and rib...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"