Anti-Measles nucleoprotein antibody [3E1] (ab9397)

Overview

  • Product name
    Anti-Measles nucleoprotein antibody [3E1]
    See all Measles nucleoprotein primary antibodies
  • Description
    Mouse monoclonal [3E1] to Measles nucleoprotein
  • Specificity
    Reacts with measles NP. Does not cross-react with mumps NP. Recognises both native and recombinant yeast-derived measles NP.
  • Tested applications
    Suitable for: WB, Indirect ELISAmore details
  • Immunogen

    Recombinant full length protein.

  • Positive control
    • recombinant measles NP
  • General notes

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    PBS with 0.1% sodium azide
  • Concentration information loading...
  • Purity
    IgG fraction
  • Clonality
    Monoclonal
  • Clone number
    3E1
  • Myeloma
    NS0/1
  • Isotype
    IgG1
  • Light chain type
    unknown
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab9397 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000.
Indirect ELISA 1/1000 - 1/10000.

Target

  • Relevance
    Involved in the encapsidating of the genome in a ratio of 1 N per 6 ribonucleotides, resulting in the protection from nucleases. The nucleocapsid (NC) has an helical structure with either 12.35 or 11.64 N per turn, approximately 20nm in diameter, with a hollow central cavity approximately 5nm in diameter. The encapsidated genomic RNA is termed the NC and serves as template for transcription and replication. During replication, encapsidation by N is coupled to RNA synthesis and all replicative products are resistant to nucleases. N is released in the blood following lysis of measles infected cells, it interacts then with human FCGR2B on immune cells, inducing apoptosis and blocking inflammatory immune response. Ntail binds to a protein on human thymic epithelial cells, termed Nucleoprotein Receptor (NR), inducing growth arrest
  • Cellular localization
    Virion. Host cytoplasm.
  • Alternative names
    • Measles nucleocapsid protein antibody
    • N antibody
    • NP antibody
    • Nucleocapsid protein antibody

Images

  • Immunoblot of measles NP using monoclonal antibody 3E1. Antibody 3E1 at a concentration of 0.5 µg/ml. Lane 1 - prestained marker proteins lane 2 - recombinant measles NP.

References

This product has been referenced in:
  • Sun Q  et al. TBK1 mediates critical effects of measles virus nucleocapsid protein (MVNP) on pagetic osteoclast formation. J Bone Miner Res 29:90-102 (2014). Read more (PubMed: 23794264) »
  • Wang FM  et al. Measles virus nucleocapsid protein, a key contributor to Paget's disease, increases IL-6 expression via down-regulation of FoxO3/Sirt1 signaling. Bone 53:269-76 (2013). Read more (PubMed: 23262029) »

See all 3 Publications for this product

Customer reviews and Q&As

Thank you for your enquiry about our Measles nucleoprotein antibodies. A nucleoprotein is defined as any of a group of complexes composed of protein and nucleic acid and found in the nuclei and cytoplasm of all living cells, as in chromatin and rib...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up