Anti-Measles phosphoprotein antibody [9H4] (ab43820)

Overview

  • Product name
    Anti-Measles phosphoprotein antibody [9H4]
  • Description
    Mouse monoclonal [9H4] to Measles phosphoprotein
  • Tested applications
    Suitable for: ELISA, WB, ICC/IFmore details
  • Species reactivity
    Measles virus. Does not react with other viruses.
  • Immunogen

    Recombinant phosphoprotein.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Constituents: 0.75% Glycine, 1.21% Tris, 2% Sucrose
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    9H4
  • Isotype
    IgG1
  • Light chain type
    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab43820 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/2000 - 1/10000.
WB 1/200 - 1/1000. Predicted molecular weight: 70 kDa.
ICC/IF 1/100 - 1/500.

Target

  • Relevance
    Measles virus belongs to the Paramyxoviridae family within the Mononegavirales order. Measles phosphoprotein is an essential component of the RNA polymerase and the nascent chain assembly complex. The non-segmented, single stranded, negative sense RNA genome of the virus is encapsidated by the nucleoprotein (N) to form a helical nucleocapsid. This ribonucleoproteic complex is the substrate for both transcription and replication. The RNA-dependent RNA polymerase binds to the nucleocapsid template via its co-factor, the phosphoprotein (P).
  • Alternative names
    • measles virus antibody
    • subacute sclerose panencephalitis virus antibody

References for Anti-Measles phosphoprotein antibody [9H4] (ab43820)

This product has been referenced in:
  • Chung HK  et al. Tunable and reversible drug control of protein production via a self-excising degron. Nat Chem Biol 11:713-20 (2015). Read more (PubMed: 26214256) »
  • Krumm SA  et al. The measles virus nucleocapsid protein tail domain is dispensable for viral polymerase recruitment and activity. J Biol Chem 288:29943-53 (2013). WB . Read more (PubMed: 24003217) »

See all 2 Publications for this product

Product Wall

The monoclonal antibody ab43820 is raised against full-length recombinant phosphoprotein, but the single epitope it recognizes has not been mapped, so we cannot predict whether the mutations you have introduced will interfere with detection by ab43820....

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Thank you for your inquiry.

Please excuse the delay in my answer. I was waiting for more in formation from the source of this antibody.

They now confirmed that ab43820 binds to the phosphorylated protein only.

The un-phosp...

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Thank you for contacting us.

The antibody ab43820 is against phophorylated form of protein. I would suggest other antibodiers in case customer is interested in detecting unphosphorylated form of measles.

ab23974; ab106292; ab101015...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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