Anti-MECR antibody - C-terminal (ab156268)

Overview

  • Product name
    Anti-MECR antibody - C-terminal
    See all MECR primary antibodies
  • Description
    Rabbit polyclonal to MECR - C-terminal
  • Host species
    Rabbit
  • Specificity
    At least two isoforms of MECR are known to exist; ab156268 will detect both isoforms.
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to 15 amino acids near the C-terminus of Human MECR (NP_057095).

  • Positive control
    • Human brain tissue lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab156268 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 40 kDa.

Target

  • Function
    Catalyzes the reduction of trans-2-enoyl-CoA to acyl-CoA with chain length from C6 to C16 in an NADPH-dependent manner with preference to medium chain length substrate. May have a role in the mitochondrial synthesis of fatty acids.
  • Tissue specificity
    Highly expressed in skeletal and heart muscle. Expressed at lower level in placenta, liver, kidney and pancreas. Weakly or not expressed in lung.
  • Sequence similarities
    Belongs to the zinc-containing alcohol dehydrogenase family. Quinone oxidoreductase subfamily.
  • Cellular localization
    Mitochondrion.
  • Information by UniProt
  • Database links
  • Alternative names
    • AI195831 antibody
    • CGI 63 antibody
    • FASN2B antibody
    • Homolog of yeast 2 enoyl thioester reductase antibody
    • HsNrbf-1 antibody
    • HsNrbf1 antibody
    • mecr antibody
    • MECR_HUMAN antibody
    • Mitochondrial 2 enoyl thioester reductase antibody
    • mitochondrial antibody
    • NRBF 1 antibody
    • NRBF-1 antibody
    • NRBF1 antibody
    • Nuclear receptor binding factor 1 antibody
    • Nuclear receptor-binding factor 1 antibody
    • OTTMUSP00000009996 antibody
    • RP23-13A13.1 antibody
    • Trans 2 enoyl CoA reductase, mitochondrial antibody
    • Trans-2-enoyl-CoA reductase antibody
    see all

Images

  • Western Blot of human, mouse and rat brain tissue lysate labeling MECR with Anti-MECR antibody - C-terminal (ab156268) at 1µg/ml.
  • Lane 1 : Anti-MECR antibody - C-terminal (ab156268) at 1 µg/ml
    Lane 2 : Anti-MECR antibody - C-terminal (ab156268) at 2 µg/ml

    All lanes : Human brain tissue lysate

    Lysates/proteins at 15 µg per lane.

    Predicted band size: 40 kDa

References

ab156268 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Heart (Embryonic))
Permeabilization
Yes - Tween-20
Specification
Heart (Embryonic)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Aug 23 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (Heart)
Gel Running Conditions
Reduced Denaturing (4%-12%)
Loading amount
20 µg
Specification
Heart
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jul 19 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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