This antibody gave a positive signal in the following whole cell lysates: HepG2; HEK293; HeLa; Jurkat; MCF7; Caco2.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40 Preservative: 0.02% Sodium azide Constituent: PBS Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use with paraformaldehyde fixed cells.
Use a concentration of 1 µg/ml. Detects a band of approximately 27 kDa (predicted molecular weight: 16 kDa).
FunctionComponent of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
Sequence similaritiesBelongs to the Mediator complex subunit 10 family.
Mediator of RNA polymerase II transcription subunit 10 antibody
Mediator of RNA polymerase II transcription, subunit 10 homolog antibody
Transformation-related gene 17 protein antibody
Transformation-related gene 20 protein antibody
Transformation-related protein 17 antibody
Transformation-related protein 20 antibody
Anti-MED10 antibody images
Immunocytochemistry/ Immunofluorescence - Anti-MED10 antibody (ab110786)This image is courtesy of an abreview submitted by Dr. Kirk Mcmanus, Univ. of Manitoba/ Cancer Care MICB
ab110786 (1/200) staining MED10 in HeLa cells (green). Cells were fixed in paraformaldehyde, permeabilised in 0.5% Triton X100/ PBS and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details, please see Abreview.
Exposure time : 4 minutesThis blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab110786 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
References for Anti-MED10 antibody (ab110786)
has not yet been referenced specifically in any publications.
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