• Product nameAnti-MED12 antibody
    See all MED12 primary antibodies
  • Description
    Rabbit polyclonal to MED12
  • Tested applicationsSuitable for: IHC-P, ICC/IF, IP, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Orangutan
  • Immunogen

    Synthetic peptide, corresponding to a region between C terminal amino acids 2150-2212 of Human MED12 (NP_005111.1)

  • Positive control
    • Whole cell lysate from HeLa, 293T and NIH 3T3 cells. IF: HeLa cell line



Our Abpromise guarantee covers the use of ab70842 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 µg/ml.
IP Use at 2-5 µg/mg of lysate.
WB 1/2000 - 1/10000. Predicted molecular weight: 227 kDa.


  • FunctionComponent of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. This subunit may specifically regulate transcription of targets of the Wnt signaling pathway and SHH signaling pathway.
  • Tissue specificityUbiquitous.
  • Involvement in diseaseDefects in MED12 are the cause of Opitz-Kaveggia syndrome (OKS) [MIM:305450]; also known as FG syndrome type 1 (FGS1) or FG syndrome (FGS). OKS is an X-linked disorder characterized by mental retardation, relative macrocephaly, hypotonia and constipation.
    Defects in MED12 are the cause of Lujan-Fryns syndrome (LUJFRYS) [MIM:309520]; also known as X-linked mental retardation with marfanoid habitus. Clinically, Lujan-Fryns syndrome can be distinguished from Opitz-Kaveggia syndrome by tall stature, hypernasal voice, hyperextensible digits and high nasal root.
  • Sequence similaritiesBelongs to the Mediator complex subunit 12 family.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Activator recruited cofactor 240 kDa component antibody
    • Activator-recruited cofactor 240 kDa component antibody
    • ARC240 antibody
    • CAG repeat protein 45 antibody
    • CAGH45 antibody
    • HOPA antibody
    • KIAA0192 antibody
    • MED12 antibody
    • MED12_HUMAN antibody
    • Mediator complex subunit 12 antibody
    • Mediator of RNA polymerase II transcription subunit 12 antibody
    • OPA containing protein antibody
    • OPA-containing protein antibody
    • Thyroid hormone receptor associated protein complex 230 kDa component antibody
    • Thyroid hormone receptor-associated protein complex 230 kDa component antibody
    • TNRC11 antibody
    • Trap230 antibody
    • Trinucleotide repeat containing gene 11 protein antibody
    • Trinucleotide repeat-containing gene 11 protein antibody
    see all

Anti-MED12 antibody images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling MED12 with ab70842 at 1/1000 (1µg/ml). Detection: DAB.
  • All lanes : Anti-MED12 antibody (ab70842) at 0.1 µg/ml

    Lane 1 : Whole cell lysate from HeLa cells at 50 µg
    Lane 2 : Whole cell lysate from HeLa cells at 15 µg
    Lane 3 : Whole cell lysate from HeLa cells at 5 µg
    Lane 4 : Whole cell lysate from 293T cells at 50 µg
    Lane 5 : Whole cell lysate from NIH 3T3 cells at 50 µg

    developed using the ECL technique

    Predicted band size : 227 kDa
    Observed band size : 238 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 65 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 3 minutes
  • Detection of Human MED12 by Immunoprecipitation using Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab70842 for IP at 3 µg/mg lysate. Subsequent WB detection was performed using ab70842 at 1 µg/ml.
  • ICC/IF image of ab70842 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab70842, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-MED12 antibody (ab70842)

This product has been referenced in:
  • Tsutsui T  et al. Mediator complex recruits epigenetic regulators via its two cyclin-dependent kinase subunits to repress transcription of immune response genes. J Biol Chem 288:20955-65 (2013). Read more (PubMed: 23749998) »
  • Chaturvedi CP  et al. Maintenance of gene silencing by the coordinate action of the H3K9 methyltransferase G9a/KMT1C and the H3K4 demethylase Jarid1a/KDM5A. Proc Natl Acad Sci U S A 109:18845-50 (2012). WB ; Mouse . Read more (PubMed: 23112189) »

See all 2 Publications for this product

Product Wall

Application Western blot
Loading amount 15 µg
Gel Running Conditions Non-reduced Non-Denaturing (Native) (4-12% Bis-Tris Gel, MES running buffer)
Sample Mouse Tissue lysate - other (Mouse whole brain tissue lysate)
Specification Mouse whole brain tissue lysate
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

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Submitted Oct 30 2013

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Heat-inactivated normal donkey serum in 0.05% PBS-T as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Antigen retrieval step None
Sample Mouse Tissue sections (Mouse, whole brain sections, striatum, cortex)
Specification Mouse, whole brain sections, striatum, cortex
Permeabilization Yes - Tween-20
Fixative Paraformaldehyde

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Submitted Oct 14 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - nuclear (293)
Loading amount 10 µg
Specification 293
Gel Running Conditions Reduced Denaturing (6%)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C

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Verified customer

Submitted Mar 01 2013