Anti-MED13/GCT antibody (ab229717)
Key features and details
- Rabbit polyclonal to MED13/GCT
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-MED13/GCT antibody
See all MED13/GCT primary antibodies -
Description
Rabbit polyclonal to MED13/GCT -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Recombinant fragment within Human MED13/GCT (internal sequence). The exact sequence is proprietary.
Database link: Q9UHV7 -
Positive control
- WB: HEK-293T, A431, HeLa and HepG2 whole cell lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab229717 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 239 kDa.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 239 kDa. |
Target
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Relevance
MED13 is a member of the mediator complex that can act as both a positive and negative regulator of transcription. The mediator complex interacts with RNA pol II and its associated proteins, the general transcription factors. Mediator proteins serve as a bridge connecting the activator and basal transcription machinery. -
Cellular localization
Nuclear -
Database links
- Entrez Gene: 9969 Human
- Entrez Gene: 327987 Mouse
- Entrez Gene: 303403 Rat
- Omim: 603808 Human
- SwissProt: Q9UHV7 Human
- SwissProt: Q5SWW4 Mouse
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Alternative names
- 1110067M05Rik antibody
- Activator-recruited cofactor 250 kDa component antibody
- ARC250 antibody
see all
Images
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All lanes : Anti-MED13/GCT antibody (ab229717) at 1/500 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell lysate
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 239 kDa5% SDS-PAGE gel.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab229717 has not yet been referenced specifically in any publications.