This fast track antibody is not yet fully characterized. It is subject to these terms and conditions



  • Form
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
  • Isotype
  • Research areas


  • Application notes
    This antibody gave a positive result in ELISA against the immunizing peptide (ab60790).
    IF/ICC: see figure below
    Not yet tested in other applications.

  • Target

    • Relevance
      MED13 is a member of the mediator complex that can act as both a positive and negative regulator of transcription. The mediator complex interacts with RNA pol II and its associated proteins, the general transcription factors. Mediator proteins serve as a bridge connecting the activator and basal transcription machinery.
    • Cellular localization
    • Database links
    • Alternative names
      • 1110067M05Rik antibody
      • Activator-recruited cofactor 250 kDa component antibody
      • ARC250 antibody
      • D030023K18 antibody
      • DRIP 250 antibody
      • DRIP250 antibody
      • HSPC221 antibody
      • KIAA0593 antibody
      • Mediator complex subunit 13 antibody
      • Mediator of RNA polymerase II transcription subunit 13 homolog antibody
      • Mediator of RNA polymerase II transcription, subunit 13 antibody
      • OTTMUSP00000001175 antibody
      • RP23-50E4.3 antibody
      • THRAP1 antibody
      • Thyroid hormone receptor associated protein 240 kDa subunit antibody
      • Thyroid hormone receptor associated protein complex component TRAP240 antibody
      • Thyroid hormone receptor-associated protein 1 antibody
      • Thyroid hormone receptor-associated protein complex 240 kDa component antibody
      • Trap240 antibody
      • Vitamin D3 receptor-interacting protein complex component DRIP250 antibody
      see all

    Anti-MED13 antibody images

    This Fast-Track antibody is not yet fully characterised. These images represent inconclusive preliminary data.

    • ICC/IF image of ab49468 stained HeLa cells. The cells were 4% PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab49468, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in Hek, Hep and MCF7 cells fixed in PFA and HeLa, Hek293, HepG2 and MCF7 fixed in 100% methanol, all at 5ug/ml. However, this Fast-Track antibody is not yet fully characterised. This image represents inconclusive preliminary data.

    References for Anti-MED13 antibody (ab49468)

    ab49468 has not yet been referenced specifically in any publications.

    Product Wall

    Yes you are correct. BSA, goat serum and glycine are diluted in PBS. I hope this helps. Please let me know if you need anything further.

    Thank you for your enquiry. ab49468 is considered a "Fast track" antibody. Fast track antibodies have only minimal characterization and as such are offered at a reduced price compared to our typical primary antibodies. ab49468 has been tested...

    Read More


    Sign up