This antibody gave a positive result in ELISA against the immunizing peptide (ab60790).
IF/ICC: see figure below
Not yet tested in other applications.
MED13 is a member of the mediator complex that can act as both a positive and negative regulator of transcription. The mediator complex interacts with RNA pol II and its associated proteins, the general transcription factors. Mediator proteins serve as a bridge connecting the activator and basal transcription machinery.
ICC/IF image of ab49468 stained HeLa cells. The cells were 4% PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab49468, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in Hek, Hep and MCF7 cells fixed in PFA and HeLa, Hek293, HepG2 and MCF7 fixed in 100% methanol, all at 5ug/ml. However, this Fast-Track antibody is not yet fully characterised. This image represents inconclusive preliminary data.
has not yet been referenced specifically in any publications.
Publishing research using ab49468? Please let us know so that we can cite the reference in this datasheet.
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