Anti-MED13/GCT antibody (ab76923)
Key features and details
- Rabbit polyclonal to MED13/GCT
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-MED13/GCT antibody
See all MED13/GCT primary antibodies -
Description
Rabbit polyclonal to MED13/GCT -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Chimpanzee, Gorilla -
Immunogen
Synthetic peptide within Human MED13/GCT aa 300-400. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. NP_005112.2
Database link: Q9UHV7 -
Positive control
- HeLa and 293T whole cell lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab76923 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 240 kDa.
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IP |
Use at 2-5 µg/mg of lysate.
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Notes |
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WB
1/2000 - 1/10000. Predicted molecular weight: 240 kDa. |
IP
Use at 2-5 µg/mg of lysate. |
Target
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Relevance
MED13 is a member of the mediator complex that can act as both a positive and negative regulator of transcription. The mediator complex interacts with RNA pol II and its associated proteins, the general transcription factors. Mediator proteins serve as a bridge connecting the activator and basal transcription machinery. -
Cellular localization
Nuclear -
Database links
- Entrez Gene: 9969 Human
- Omim: 603808 Human
- SwissProt: Q9UHV7 Human
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Alternative names
- 1110067M05Rik antibody
- Activator-recruited cofactor 250 kDa component antibody
- ARC250 antibody
see all
Images
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All lanes : Anti-MED13/GCT antibody (ab76923) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Predicted band size: 240 kDa
Observed band size: 300 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutes -
Detection of MED13/GCT by Western Blot of Immunprecipitate.
ab76923 at 1µg/ml staining MED13/GCT in HeLa whole cell lysate immunoprecipitated using ab76923 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 30 seconds.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab76923 has been referenced in 4 publications.
- Tripathi S et al. Smad7:ß-catenin complex regulates myogenic gene transcription. Cell Death Dis 10:387 (2019). PubMed: 31097718
- Snijders Blok L et al. De novo mutations in MED13, a component of the Mediator complex, are associated with a novel neurodevelopmental disorder. Hum Genet N/A:N/A (2018). PubMed: 29740699
- Vijayalingam S & Chinnadurai G Adenovirus L-E1A activates transcription through mediator complex-dependent recruitment of the super elongation complex. J Virol 87:3425-34 (2013). Human . PubMed: 23302885
- Fukuda A et al. Heterogeneous nuclear ribonucleoprotein R cooperates with mediator to facilitate transcription reinitiation on the c-Fos gene. PLoS One 8:e72496 (2013). PubMed: 23967313