Overview

  • Product nameAnti-MED13 antibody
    See all MED13 primary antibodies
  • Description
    Rabbit polyclonal to MED13
  • Tested applicationsSuitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Gorilla
  • Immunogen

    Synthetic peptide corresponding to a region between residue 325 and 375 of human MED13 (NP_005112.2)

  • Positive control
    • HeLa and 293T whole cell lysates.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab76923 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 240 kDa.
IP Use at 2-5 µg/mg of lysate.

Target

  • RelevanceMED13 is a member of the mediator complex that can act as both a positive and negative regulator of transcription. The mediator complex interacts with RNA pol II and its associated proteins, the general transcription factors. Mediator proteins serve as a bridge connecting the activator and basal transcription machinery.
  • Cellular localizationNuclear
  • Database links
  • Alternative names
    • 1110067M05Rik antibody
    • Activator-recruited cofactor 250 kDa component antibody
    • ARC250 antibody
    • D030023K18 antibody
    • DRIP 250 antibody
    • DRIP250 antibody
    • HSPC221 antibody
    • KIAA0593 antibody
    • Mediator complex subunit 13 antibody
    • Mediator of RNA polymerase II transcription subunit 13 homolog antibody
    • Mediator of RNA polymerase II transcription, subunit 13 antibody
    • OTTMUSP00000001175 antibody
    • RP23-50E4.3 antibody
    • THRAP1 antibody
    • Thyroid hormone receptor associated protein 240 kDa subunit antibody
    • Thyroid hormone receptor associated protein complex component TRAP240 antibody
    • Thyroid hormone receptor-associated protein 1 antibody
    • Thyroid hormone receptor-associated protein complex 240 kDa component antibody
    • Trap240 antibody
    • Vitamin D3 receptor-interacting protein complex component DRIP250 antibody
    see all

Anti-MED13 antibody images

  • All lanes : Anti-MED13 antibody (ab76923) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg


    Predicted band size : 240 kDa
    Observed band size : 300 kDa (why is the actual band size different from the predicted?)


    Exposure time : 3 minutes
  • Detection of MED13 by Western Blot of Immunprecipitate.
    ab76923 at 1µg/ml staining MED13 in HeLa whole cell lysate immunoprecipitated using ab76923 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: Chemiluminescence with exposure time of 30 seconds.

References for Anti-MED13 antibody (ab76923)

This product has been referenced in:
  • Vijayalingam S & Chinnadurai G Adenovirus L-E1A activates transcription through mediator complex-dependent recruitment of the super elongation complex. J Virol 87:3425-34 (2013). Human . Read more (PubMed: 23302885) »
  • Fukuda A  et al. Heterogeneous nuclear ribonucleoprotein R cooperates with mediator to facilitate transcription reinitiation on the c-Fos gene. PLoS One 8:e72496 (2013). Read more (PubMed: 23967313) »

See all 2 Publications for this product

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I am sorry to confirm that we are regrettably not able to provide this particular antibody BSA and azide free. We do however have some purification kits available on our catalog which I hope may be helpful. This c...

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