Overview

  • Product name
  • Description
    Rabbit polyclonal to MED18
  • Tested applications
    Suitable for: IP, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Zebrafish, Rhesus monkey, Gorilla, Catfish, Orangutan, Platypus
  • Immunogen

    Synthetic peptide corresponding to Human MED18 (C terminal). A region between residue 150 and the C-terminus (residue 208) of human MED18 using the numbering given in entry NP_060108.1 (GeneID 54797).

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab71556 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/2000 - 1/10000. Predicted molecular weight: 24 kDa.

Target

  • Function
    Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
  • Sequence similarities
    Belongs to the Mediator complex subunit 18 family.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ20045 antibody
    • hMED 18 antibody
    • hMED18 antibody
    • Human Mediator of RNA Polymerase II Transcription antibody
    • MED 18 antibody
    • med18 antibody
    • MED18_HUMAN antibody
    • Mediator complex subunit 18 antibody
    • Mediator of RNA polymerase II transcription subunit 18 antibody
    • Mediator of RNA polymerase II transcription subunit 18 homolog antibody
    • OTTHUMP00000003645 antibody
    • p28b antibody
    • TRAP/mediator complex subunit p28b antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling MED18 with ab71556 at 1/200 (1µg/ml). Detection: DAB.
  • All lanes : Anti-MED18 antibody (ab71556) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg
    Lane 5 : Mouse NIH3T3 whole cell lysate at 50 µg


    Predicted band size : 24 kDa


    Exposure time : 3 minutes
  • MED18 antibody (ab71556) at 3 µg/mg lysate, staining human MED18 in HeLa whole cell lysate by immunoprecipitation. 1 mg of HeLa whole cell lysate was used, 20% of IP loaded. Detection was by chemiluminescence with 30 second exposure.

References

ab71556 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab71556.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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