Overview

  • Product name
  • Description
    Rabbit polyclonal to MED18
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Zebrafish, Rhesus monkey, Gorilla, Catfish, Orangutan, Platypus
  • Immunogen

    Synthetic peptide, corresponding to a region within the C terminal amino acids 150-208 of Human MED18, NP_060108.1

  • Positive control
    • Human prostate adenocarcinoma. Mouse squamous cell carcinoma.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab84431 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
  • Application notes
    IHC-P: 1/100 - 1/500. Epitope exposure is recommended. Epitope exposure with citrate buffer will enhance staining.

    Likely to work with frozen sections.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
    • Sequence similarities
      Belongs to the Mediator complex subunit 18 family.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • FLJ20045 antibody
      • hMED 18 antibody
      • hMED18 antibody
      • Human Mediator of RNA Polymerase II Transcription antibody
      • MED 18 antibody
      • med18 antibody
      • MED18_HUMAN antibody
      • Mediator complex subunit 18 antibody
      • Mediator of RNA polymerase II transcription subunit 18 antibody
      • Mediator of RNA polymerase II transcription subunit 18 homolog antibody
      • OTTHUMP00000003645 antibody
      • p28b antibody
      • TRAP/mediator complex subunit p28b antibody
      see all

    Images

    • Detection of Human MED18 by Immunohistochemistry.
      Sample: FFPE section of Human prostate adenocarcinoma.
      Antibody: ab84431 used at a dilution of 1/250.
      Detection: DAB staining.
    • Detection of Mouse MED18 by Immunohistochemistry.
      Sample: FFPE section of Mouse squamous cell carcinoma.
      Antibody: ab84431 used at a dilution of 1/250.
      Detection: DAB staining.

    References

    ab84431 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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