Overview

  • Product name
  • Description
    Rabbit polyclonal to MED25
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to N terminal amino acids 119-148 of Human MED25 (NP_112235.2).

  • Positive control
    • Mouse kidney tissue lysates; MCF7 cell line lysates.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    This antibody is purified through a protein A column, followed by peptide affinity purification.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab107551 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 78 kDa.

Target

  • Function
    Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Required for RARA/RXRA-mediated transcription.
  • Tissue specificity
    Ubiquitously expressed. Highest levels in brain, heart, kidney, peripheral leukocytes, placenta, skeletal muscle and spleen.
  • Involvement in disease
    Defects in MED25 are the cause of Charcot-Marie-Tooth disease type 2B2 (CMT2B2) [MIM:605589]. It is a recessive axonal form of Charcot-Marie-Tooth disease, a disorder of the peripheral nervous system, characterized by progressive weakness and atrophy, initially of the peroneal muscles and later of the distal muscles of the arms. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathies(designated CMT1 when they are dominantly inherited) and primary peripheral axonal neuropathies (CMT2). Neuropathies of the CMT2 group are characterized by signs of axonal regeneration in the absence of obvious myelin alterations, normal or slightly reduced nerve conduction velocities, and progressive distal muscle weakness and atrophy. Nerve conduction velocities are normal or slightly reduced.
  • Sequence similarities
    Belongs to the Mediator complex subunit 25 family.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • ACID1 antibody
    • Activator interaction domain 1 protein antibody
    • Activator interaction domain-containing protein 1 antibody
    • Activator-recruited cofactor 92 kDa component antibody
    • ARC/mediator transcriptional coactivator subunit antibody
    • ARC/mediator transcriptional coactivator subunit ARC92 antibody
    • ARC92 antibody
    • BVSYS antibody
    • CMT2B2 antibody
    • DKFZp434K0512 antibody
    • FLJ10193 antibody
    • med25 antibody
    • MED25 protein antibody
    • MED25_HUMAN antibody
    • Mediator complex subunit 25 antibody
    • Mediator of RNA polymerase II transcription subunit 25 antibody
    • Mediator of RNA polymerase II transcription, subunit 25 homolog (S cerevisiae) antibody
    • Mediator of RNA polymerase II transcription, subunit 25 homolog (yeast) antibody
    • MGC70671 antibody
    • p78 antibody
    • prostate-derived protein 78 kDa antibody
    • PTOV2 antibody
    • TCBAP0758 antibody
    see all

Images

  • Anti-MED25 antibody (ab107551) at 1/100 dilution + Mouse kidney tissue lysates at 35 µg

    Predicted band size : 78 kDa
  • Anti-MED25 antibody (ab107551) at 1/100 dilution + MCF7 cell line lysates at 35 µg

    Predicted band size : 78 kDa

References

ab107551 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - nuclear (Mouse embryonic Stem Cells)
Gel Running Conditions
Reduced Denaturing (12% gel)
Loading amount
35 µg
Specification
Mouse embryonic Stem Cells
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C
Username

Jan-Aurel Pfister

Verified customer

Submitted Apr 10 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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