Overview

  • Product nameAnti-MED31 antibody
    See all MED31 primary antibodies
  • Description
    Mouse monoclonal to MED31
  • Tested applicationsSuitable for: WB, IHC-P, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein, corresponding to amino acids 1-132 of Human MED31

Properties

Applications

Our Abpromise guarantee covers the use of ab54761 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 16 kDa.
IHC-P Use a concentration of 3 µg/ml.
Flow Cyt Use 1µg for 106 cells. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

Target

  • RelevanceMED31 is a member of the mediator complex that can act as both a positive and negative regulator of transcription. The mediator complex interacts with RNA pol II and its associated proteins, the general transcription factors. Mediator proteins serve as a bridge connecting the activator and basal transcription machinery.
  • Cellular localizationNuclear
  • Database links
  • Alternative names
    • SOH1 antibody
    • CGI-125 antibody
    • hSOH1 antibody
    • Mediator complex subunit 31 antibody
    • Mediator complex subunit SOH1 antibody
    • Mediator of RNA polymerase II transcription subunit 31 antibody
    see all

Anti-MED31 antibody images

  • MED31 antibody (ab54761) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human placenta.


  • Predicted band size : 16 kDa
    MED31 antibody (ab54761) at 1ug/lane + MCF-7 cell lysate at 25ug/lane.
  • Overlay histogram showing HeLa cells stained with ab54761 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab54761, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

References for Anti-MED31 antibody (ab54761)

This product has been referenced in:
  • Vijayalingam S & Chinnadurai G Adenovirus L-E1A activates transcription through mediator complex-dependent recruitment of the super elongation complex. J Virol 87:3425-34 (2013). Human . Read more (PubMed: 23302885) »
  • Tsai KL  et al. A conserved Mediator-CDK8 kinase module association regulates Mediator-RNA polymerase II interaction. Nat Struct Mol Biol 20:611-9 (2013). WB ; Human . Read more (PubMed: 23563140) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"