The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 16 kDa.
Use a concentration of 3 µg/ml.
Use 1µg for 106 cells. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
RelevanceMED31 is a member of the mediator complex that can act as both a positive and negative regulator of transcription. The mediator complex interacts with RNA pol II and its associated proteins, the general transcription factors. Mediator proteins serve as a bridge connecting the activator and basal transcription machinery.
Mediator of RNA polymerase II transcription subunit 31 antibody
Anti-MED31 antibody images
IHC-P - MED31 antibody (ab54761)
MED31 antibody (ab54761) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human placenta.
Western blot - MED31 antibody (ab54761)
Predicted band size : 16 kDa MED31 antibody (ab54761) at 1ug/lane + MCF-7 cell lysate at 25ug/lane.
Flow Cytometry-Anti-MED31 antibody(ab54761)
Overlay histogram showing HeLa cells stained with ab54761 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab54761, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
References for Anti-MED31 antibody (ab54761)
This product has been referenced in:
Vijayalingam S & Chinnadurai G Adenovirus L-E1A activates transcription through mediator complex-dependent recruitment of the super elongation complex. J Virol87:3425-34 (2013).
Read more (PubMed: 23302885) »
Tsai KL et al. A conserved Mediator-CDK8 kinase module association regulates Mediator-RNA polymerase II interaction. Nat Struct Mol Biol20:611-9 (2013).
Read more (PubMed: 23563140) »