The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 16 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Use at an assay dependent concentration.
RelevanceMED31 is a member of the mediator complex that can act as both a positive and negative regulator of transcription. The mediator complex interacts with RNA pol II and its associated proteins, the general transcription factors. Mediator proteins serve as a bridge connecting the activator and basal transcription machinery.
Mediator of RNA polymerase II transcription subunit 31 antibody
Anti-MED31 antibody - ChIP Grade images
ChIP - Anti-MED31 antibody (ab98142)
Quiescent human colon carcinoma HCT116 cultures were treated with 10% FBS for three time points (0, 15, 30 minutes) or (0, 30, 60 minutes) were used in Matrix-ChIP and real-time PCR assays at EGR1 gene (Exon1) and 15kb upstream site.
Western blot - MED31 antibody (ab98142)
Anti-MED31 antibody - ChIP Grade (ab98142) at 1 µg/ml (5% skim milk / PBS buffer) + Human fetal liver lysate at 10 µg