• Product nameAnti-MED6 antibody
    See all MED6 primary antibodies
  • Description
    Rabbit polyclonal to MED6
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Cow, Cynomolgus monkey
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 200 to the C-terminus of Human MED6.

    (Peptide available as ab35431.)

  • Positive control
    • A431 (Human epithelial carcinoma cell line) Whole Cell Lysate



Our Abpromise guarantee covers the use of ab35432 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 28 kDa).


  • FunctionComponent of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
  • Sequence similaritiesBelongs to the Mediator complex subunit 6 family.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Activator recruited cofactor 33 kDa antibody
    • Activator-recruited cofactor 33 kDa component antibody
    • ARC33 antibody
    • hMed6 antibody
    • MED6 antibody
    • MED6_HUMAN antibody
    • Mediator complex subunit 6 antibody
    • Mediator of RNA polymerase II transcription subunit 6 antibody
    • NY REN 28 antigen antibody
    • Renal carcinoma antigen NY-REN-28 antibody
    • RNA polymerase transcriptional regulation mediator subunit 6 antibody
    see all

Anti-MED6 antibody images

  • Lane 1 : Marker
    Lanes 2 - 4 : Anti-MED6 antibody (ab35432) at 1 µg/ml

    Lane 1 : As above
    Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
    Lane 3 : Jurkat whole cell lysate (ab7899) at 20 µg
    Lane 4 : A431 whole cell lysate (ab7909) at 20 µg

    Lanes 2 - 4 : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution

    Performed under reducing conditions.

    Predicted band size : 28 kDa
    Observed band size : 32 kDa (why is the actual band size different from the predicted?)

References for Anti-MED6 antibody (ab35432)

This product has been referenced in:
  • Ang K  et al. Mediator acts upstream of the transcriptional activator Gal4. PLoS Biol 10:e1001290 (2012). WB ; Human . Read more (PubMed: 22479149) »
  • Zhou H  et al. MED12 mutations link intellectual disability syndromes with dysregulated GLI3-dependent Sonic Hedgehog signaling. Proc Natl Acad Sci U S A 109:19763-8 (2012). WB . Read more (PubMed: 23091001) »

See all 2 Publications for this product

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