Overview

  • Product nameAnti-MED6 antibody
    See all MED6 primary antibodies
  • Description
    Rabbit polyclonal to MED6
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Cow, Cynomolgus Monkey
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 200 to the C-terminus of Human MED6.

    (Peptide available as ab35431.)

  • Positive control
    • A431 (Human epithelial carcinoma cell line) Whole Cell Lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab35432 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 28 kDa).

Target

  • FunctionComponent of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
  • Sequence similaritiesBelongs to the Mediator complex subunit 6 family.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Activator recruited cofactor 33 kDa antibody
    • Activator-recruited cofactor 33 kDa component antibody
    • ARC33 antibody
    • hMed6 antibody
    • MED6 antibody
    • MED6_HUMAN antibody
    • Mediator complex subunit 6 antibody
    • Mediator of RNA polymerase II transcription subunit 6 antibody
    • NY REN 28 antigen antibody
    • Renal carcinoma antigen NY-REN-28 antibody
    • RNA polymerase transcriptional regulation mediator subunit 6 antibody
    see all

Anti-MED6 antibody images

  • Lane 1 : Marker
    Lanes 2 - 4 : Anti-MED6 antibody (ab35432) at 1 µg/ml

    Lane 1 : As above
    Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
    Lane 3 : Jurkat whole cell lysate (ab7899) at 20 µg
    Lane 4 : A431 whole cell lysate (ab7909) at 20 µg

    Secondary
    Lanes 2 - 4 : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution


    Performed under reducing conditions.

    Predicted band size : 28 kDa
    Observed band size : 32 kDa (why is the actual band size different from the predicted?)

References for Anti-MED6 antibody (ab35432)

This product has been referenced in:
  • Ang K  et al. Mediator acts upstream of the transcriptional activator Gal4. PLoS Biol 10:e1001290 (2012). WB ; Human . Read more (PubMed: 22479149) »
  • Zhou H  et al. MED12 mutations link intellectual disability syndromes with dysregulated GLI3-dependent Sonic Hedgehog signaling. Proc Natl Acad Sci U S A 109:19763-8 (2012). WB . Read more (PubMed: 23091001) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"