Overview

  • Product nameAnti-MED6 antibody - ChIP Grade
    See all MED6 primary antibodies
  • Description
    Rabbit polyclonal to MED6 - ChIP Grade
  • Tested applicationsSuitable for: WB, ChIPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig
  • Immunogen

    Synthetic peptide within residues: LLLDLRQKFP PKFVQLKPGE KPVPVDQTKK EAEPIPETVK PEEKETTKNV, corresponding to internal sequence amino acids 180-229 of Human MED6 (NP_005457)

  • Positive control
    • 721_B cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab84968 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ChIP Use at an assay dependent concentration.

Target

  • FunctionComponent of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
  • Sequence similaritiesBelongs to the Mediator complex subunit 6 family.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Activator recruited cofactor 33 kDa antibody
    • Activator-recruited cofactor 33 kDa component antibody
    • ARC33 antibody
    • hMed6 antibody
    • MED6 antibody
    • MED6_HUMAN antibody
    • Mediator complex subunit 6 antibody
    • Mediator of RNA polymerase II transcription subunit 6 antibody
    • NY REN 28 antigen antibody
    • Renal carcinoma antigen NY-REN-28 antibody
    • RNA polymerase transcriptional regulation mediator subunit 6 antibody
    see all

Anti-MED6 antibody - ChIP Grade images

  • Quiescent human colon carcinoma HCT116 cultures were treated with 10% FBS for three time points (0, 15, 30 minutes) or (0, 30, 60 minutes) were used in Matrix-ChIP and real-time PCR assays at EGR1 gene (Exon1) and 15kb upstream site.

  • Anti-MED6 antibody - ChIP Grade (ab84968) at 1 µg/ml + 721_B cell lysate at 10 µg/ml

    Secondary
    anti-Rabbit IgG HRP at 1/50000 dilution

    Predicted band size : 28 kDa
    Observed band size : 28 kDa

References for Anti-MED6 antibody - ChIP Grade (ab84968)

ab84968 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"