Overview

  • Product nameAnti-MED8 antibody
    See all MED8 primary antibodies
  • Description
    Rabbit polyclonal to MED8
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to a region within internal sequence amino acids 102-130 of Human MED8 (NP_443109.2, NP_963836.2).

  • Positive control
    • HL60 cell line lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab112921 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 29 kDa.

Target

  • FunctionComponent of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. May play a role as a target recruitment subunit in E3 ubiquitin-protein ligase complexes and thus in ubiquitination and subsequent proteasomal degradation of target proteins.
  • PathwayProtein modification; protein ubiquitination.
  • Sequence similaritiesBelongs to the Mediator complex subunit 8 family.
  • DomainThe elongin BC complex binding domain is also known as BC-box with the consensus [APST]-L-x(3)-C-x(3)-[AILV].
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Activator recruited cofactor 32 kDa component antibody
    • Activator-recruited cofactor 32 kDa component antibody
    • ARC32 antibody
    • med8 antibody
    • MED8_HUMAN antibody
    • Mediator complex subunit 8 antibody
    • Mediator of RNA polymerase II transcription subunit 8 antibody
    • Mediator of RNA polymerase II transcription subunit 8 homolog antibody
    see all

Anti-MED8 antibody images

  • Anti-MED8 antibody (ab112921) at 1/100 dilution + HL60 cell line lysates at 35 µg

    Predicted band size : 29 kDa

References for Anti-MED8 antibody (ab112921)

ab112921 has not yet been referenced specifically in any publications.

Product Wall

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