Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab57703 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWB: Use at a concentration of 1-5 µg/ml.

    This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionComponent of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. May play a role as a target recruitment subunit in E3 ubiquitin-protein ligase complexes and thus in ubiquitination and subsequent proteasomal degradation of target proteins.
    • PathwayProtein modification; protein ubiquitination.
    • Sequence similaritiesBelongs to the Mediator complex subunit 8 family.
    • DomainThe elongin BC complex binding domain is also known as BC-box with the consensus [APST]-L-x(3)-C-x(3)-[AILV].
    • Cellular localizationNucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • Activator recruited cofactor 32 kDa component antibody
      • Activator-recruited cofactor 32 kDa component antibody
      • ARC32 antibody
      • med8 antibody
      • MED8_HUMAN antibody
      • Mediator complex subunit 8 antibody
      • Mediator of RNA polymerase II transcription subunit 8 antibody
      • Mediator of RNA polymerase II transcription subunit 8 homolog antibody
      see all

    Anti-MED8 antibody images

    • Western blot against tagged recombinant protein immunogen using ab57703 MED8 antibody at 1ug/ml. Predicted band size of immunogen is 33 kDa

    References for Anti-MED8 antibody (ab57703)

    ab57703 has not yet been referenced specifically in any publications.

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    There are currently no Abreviews or Questions for ab57703.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"