Overview

  • Product name
  • Description
    Rabbit polyclonal to MED8
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to a region within the N terminal amino acids 2-51 (QREEKQLEAS LDALLSQVAD LKNSLGSFIC KLENEYGRLT WPSVLDSFAL) of Human MED8 (NP_443109).

  • Positive control
    • Fetal stomach lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab87222 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 39 kDa (predicted molecular weight: 33 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function
    Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. May play a role as a target recruitment subunit in E3 ubiquitin-protein ligase complexes and thus in ubiquitination and subsequent proteasomal degradation of target proteins.
  • Pathway
    Protein modification; protein ubiquitination.
  • Sequence similarities
    Belongs to the Mediator complex subunit 8 family.
  • Domain
    The elongin BC complex binding domain is also known as BC-box with the consensus [APST]-L-x(3)-C-x(3)-[AILV].
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Activator recruited cofactor 32 kDa component antibody
    • Activator-recruited cofactor 32 kDa component antibody
    • ARC32 antibody
    • med8 antibody
    • MED8_HUMAN antibody
    • Mediator complex subunit 8 antibody
    • Mediator of RNA polymerase II transcription subunit 8 antibody
    • Mediator of RNA polymerase II transcription subunit 8 homolog antibody
    see all

Images

  • Anti-MED8 antibody (ab87222) at 1 µg/ml (5% skim milk / PBS buffer) + fetal stomach lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 33 kDa
    Observed band size : 39 kDa (why is the actual band size different from the predicted?)

References

ab87222 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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