Anti-MEF2A antibody [2F9H2] (ab181494)
Key features and details
- Mouse monoclonal [2F9H2] to MEF2A
- Suitable for: WB
- Reacts with: Human, Recombinant fragment
- Isotype: IgG1
Overview
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Product name
Anti-MEF2A antibody [2F9H2]
See all MEF2A primary antibodies -
Description
Mouse monoclonal [2F9H2] to MEF2A -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human, Recombinant fragment
Predicted to work with: Mouse, Rat, Cow, Pig, Orangutan -
Immunogen
Recombinant fragment corresponding to Human MEF2A aa 391-497. (Expressed in E.coli).
Sequence:SIKSEPISPPRDRMTPSGFQQQQQQQQQQQPPPPPQPQPQPPQPQPRQEM GRSPVDSLSSSSSSYDGSDREDPRGDFHSPIVLGRPPNTEDRESPSVKRM RMDAWVT
Database link: Q02078-6 -
Positive control
- MEF2A (AA: 391-497) recombinant protein; MEF2A (AA: 391-497)-hIgGFc transfected HEK293 cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: 99% PBS
Contains 0.5% protein stabilizer. -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
2F9H2 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab181494 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/2000. Predicted molecular weight: 55 kDa.
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Notes |
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WB
1/500 - 1/2000. Predicted molecular weight: 55 kDa. |
Target
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Function
Transcriptional activator which binds specifically to the MEF2 element, 5'-YTA[AT](4)TAR-3', found in numerous muscle-specific genes. Also involved in the activation of numerous growth factor- and stress-induced genes. Mediates cellular functions not only in skeletal and cardiac muscle development, but also in neuronal differentiation and survival. Plays diverse roles in the control of cell growth, survival and apoptosis via p38 MAPK signaling in muscle-specific and/or growth factor-related transcription. In cerebellar granule neurons, phosphorylated and sumoylated MEF2A represses transcription of NUR77 promoting synaptic differentiation. -
Tissue specificity
Isoform MEF2 and isoform MEFA are expressed only in skeletal and cardiac muscle and in the brain. Isoform RSRFC4 and isoform RSRFC9 are expressed in all tissues examined. -
Involvement in disease
Defects in MEF2A might be a cause of autosomal dominant coronary artery disease 1 with myocardial infarction (ADCAD1) [MIM:608320]. -
Sequence similarities
Belongs to the MEF2 family.
Contains 1 MADS-box domain.
Contains 1 Mef2-type DNA-binding domain. -
Post-translational
modificationsConstitutive phosphorylation on Ser-408 promotes Lys-403 sumoylation thus preventing acetylation at this site. Dephosphorylation on Ser-408 by PPP3CA upon neuron depolarization promotes a switch from sumoylation to acetylation on residue Lys-403 leading to inhibition of dendrite claw differentiation. Phosphorylation on Thr-312 and Thr-319 are the main sites involved in p38 MAPK signaling and activate transcription. Phosphorylated on these sites by MAPK14/p38alpha and MAPK11/p38beta, but not by MAPK13/p38delta nor by MAPK12/p38gamma. Phosphorylation on Ser-408 by CDK5 induced by neurotoxicity inhibits MEF2A transcriptional activation leading to apoptosis of cortical neurons. Phosphorylation on Thr-312, Thr-319 and Ser-355 can be induced by EGF.
Sumoylation on Lys-403 is enhanced by PIAS1 and represses transcriptional activity. Phosphorylation on Ser-408 is required for sumoylation. Has no effect on nuclear location nor on DNA binding. Sumoylated by SUMO1 and, to a lesser extent by SUMO2 and SUMO3. PIASx facilitates sumoylation in postsynaptic dendrites in the cerebellar cortex and promotes their morphogenesis.
Acetylation on Lys-403 activates transcriptional activity. Acetylated by p300 on several sites in diffentiating myocytes. Acetylation on Lys-4 increases DNA binding and transactivation (By similarity). Hyperacetylation by p300 leads to enhanced cardiac myocyte growth and heart failure.
Proteolytically cleaved in cerebellar granule neurons on several sites by caspase 3 and caspase 7 following neurotoxicity. Preferentially cleaves the CDK5-mediated hyperphosphorylated form which leads to neuron apoptosis and transcriptional inactivation. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 407231 Cow
- Entrez Gene: 4205 Human
- Entrez Gene: 17258 Mouse
- Entrez Gene: 100037273 Pig
- Entrez Gene: 309957 Rat
- Omim: 600660 Human
- SwissProt: A2VDZ3 Cow
- SwissProt: Q02078 Human
see all -
Alternative names
- ADCAD1 antibody
- MADS box transcription enhancer factor 2, polypeptide A (myocyte enhancer factor 2A) antibody
- MEF2 antibody
see all
Images
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All lanes : Anti-MEF2A antibody [2F9H2] (ab181494) at 1/500 dilution
Lane 1 : HEK293 cell lysate
Lane 2 : MEF2A (AA: 391-497)-hIgGFc transfected HEK293 cell lysate
Predicted band size: 55 kDa -
Anti-MEF2A antibody [2F9H2] (ab181494) at 1/500 dilution + MEF2A (AA: 391-497) recombinant protein
Predicted band size: 55 kDaExpected MWt is 38 kDa.
Datasheets and documents
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Datasheet download
References (1)
ab181494 has been referenced in 1 publication.
- Wu L et al. Methionine restriction at the post-weanling period promotes muscle fiber transition in piglets and improves intramuscular fat content in growing-finishing pigs. Amino Acids 51:1657-1666 (2019). PubMed: 31729551