Recombinant
RabMAb

Anti-MEF2A antibody [EP1706Y] (ab76063)

Overview

  • Product name
    Anti-MEF2A antibody [EP1706Y]
    See all MEF2A primary antibodies
  • Description
    Rabbit monoclonal [EP1706Y] to MEF2A
  • Tested applications
    Suitable for: WB, ICC, Flow Cyt, IHC-Pmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human MEF2A aa 400-500.

  • Positive control
    • WB: Fetal brain, human heart and HeLa lysates. IHC-P: Human kidney tissue
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab76063 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 55 kDa.
ICC 1/100 - 1/250.
Flow Cyt 1/20 - 1/50. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-P Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function
      Transcriptional activator which binds specifically to the MEF2 element, 5'-YTA[AT](4)TAR-3', found in numerous muscle-specific genes. Also involved in the activation of numerous growth factor- and stress-induced genes. Mediates cellular functions not only in skeletal and cardiac muscle development, but also in neuronal differentiation and survival. Plays diverse roles in the control of cell growth, survival and apoptosis via p38 MAPK signaling in muscle-specific and/or growth factor-related transcription. In cerebellar granule neurons, phosphorylated and sumoylated MEF2A represses transcription of NUR77 promoting synaptic differentiation.
    • Tissue specificity
      Isoform MEF2 and isoform MEFA are expressed only in skeletal and cardiac muscle and in the brain. Isoform RSRFC4 and isoform RSRFC9 are expressed in all tissues examined.
    • Involvement in disease
      Defects in MEF2A might be a cause of autosomal dominant coronary artery disease 1 with myocardial infarction (ADCAD1) [MIM:608320].
    • Sequence similarities
      Belongs to the MEF2 family.
      Contains 1 MADS-box domain.
      Contains 1 Mef2-type DNA-binding domain.
    • Post-translational
      modifications
      Constitutive phosphorylation on Ser-408 promotes Lys-403 sumoylation thus preventing acetylation at this site. Dephosphorylation on Ser-408 by PPP3CA upon neuron depolarization promotes a switch from sumoylation to acetylation on residue Lys-403 leading to inhibition of dendrite claw differentiation. Phosphorylation on Thr-312 and Thr-319 are the main sites involved in p38 MAPK signaling and activate transcription. Phosphorylated on these sites by MAPK14/p38alpha and MAPK11/p38beta, but not by MAPK13/p38delta nor by MAPK12/p38gamma. Phosphorylation on Ser-408 by CDK5 induced by neurotoxicity inhibits MEF2A transcriptional activation leading to apoptosis of cortical neurons. Phosphorylation on Thr-312, Thr-319 and Ser-355 can be induced by EGF.
      Sumoylation on Lys-403 is enhanced by PIAS1 and represses transcriptional activity. Phosphorylation on Ser-408 is required for sumoylation. Has no effect on nuclear location nor on DNA binding. Sumoylated by SUMO1 and, to a lesser extent by SUMO2 and SUMO3. PIASx facilitates sumoylation in postsynaptic dendrites in the cerebellar cortex and promotes their morphogenesis.
      Acetylation on Lys-403 activates transcriptional activity. Acetylated by p300 on several sites in diffentiating myocytes. Acetylation on Lys-4 increases DNA binding and transactivation (By similarity). Hyperacetylation by p300 leads to enhanced cardiac myocyte growth and heart failure.
      Proteolytically cleaved in cerebellar granule neurons on several sites by caspase 3 and caspase 7 following neurotoxicity. Preferentially cleaves the CDK5-mediated hyperphosphorylated form which leads to neuron apoptosis and transcriptional inactivation.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • ADCAD1 antibody
      • MADS box transcription enhancer factor 2, polypeptide A (myocyte enhancer factor 2A) antibody
      • MEF2 antibody
      • MEF2A antibody
      • MEF2A_HUMAN antibody
      • Myocyte enhancer factor 2A antibody
      • Myocyte-specific enhancer factor 2A antibody
      • RSRFC4 antibody
      • RSRFC9 antibody
      • Serum response factor like protein 1 antibody
      • Serum response factor-like protein 1 antibody
      see all

    Images

    • All lanes : Anti-MEF2A antibody [EP1706Y] (ab76063) at 1/1000 dilution

      Lane 1 : Murine cardiomyocyte whole cell lysate
      Lane 2 : Murine cardiomyocyte whole cell lysate
      Lane 3 : Murine cardiomyocyte whole cell lysate
      Lane 4 : Murine cardiomyocyte whole cell lysate

      Lysates/proteins at 25 µg per lane.

      Secondary
      Goat anti-rabbit polyclonal HRP conjugate.

      Performed under reducing conditions.

      Predicted band size : 55 kDa


      Exposure time : 30 seconds

      Image is courtesy of an anonymous AbReview.

      Lanes from left to right: cropped MW marker ladder, 1, 2, 3, 4.

      Blocking was performed with 5% milk for 1 hour at room temperature.

      See Abreview

    • All lanes : Anti-MEF2A antibody [EP1706Y] (ab76063) at 1/10000 dilution

      Lane 1 : Fetal brain lysate
      Lane 2 : Human heart lysate
      Lane 3 : HeLa lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 55 kDa
      Observed band size : 70 kDa (why is the actual band size different from the predicted?)
      The observed band size may be different from the predicted molecular weight as the protein is sumoylated.
    • Immunohistochemical analysis of MEF2A in paraffin embedded human kidney tissue using ab76063 at a 1/50 dilution.
    • Overlay histogram showing MCF-7 cells stained with ab76063 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab76063, 1/50 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit monoclonal IgG (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in MCF-7 cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

    References

    This product has been referenced in:
    • Belian E  et al. Forward Programming of Cardiac Stem Cells by Homogeneous Transduction with MYOCD plus TBX5. PLoS One 10:e0125384 (2015). WB, IF . Read more (PubMed: 26047103) »
    • Malek MH  et al. Similar skeletal muscle angiogenic and mitochondrial signalling following 8 weeks of endurance exercise in mice: discontinuous versus continuous training. Exp Physiol 98:807-18 (2013). Read more (PubMed: 23180811) »

    See all 2 Publications for this product

    Customer reviews and Q&As

    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Heart)
    Gel Running Conditions
    Reduced Denaturing (15% PAGE)
    Loading amount
    25 µg
    Specification
    Heart
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Username

    Abcam user community

    Verified customer

    Submitted Oct 05 2015

    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (Cardiomyocytes)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    25 µg
    Specification
    Cardiomyocytes
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Username

    Abcam user community

    Verified customer

    Submitted Sep 28 2015

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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