Recombinant
RabMAb

Anti-MEF2C [EPR1452(N)] antibody (ab191428)

Overview

  • Product name
    Anti-MEF2C [EPR1452(N)] antibody
    See all MEF2C primary antibodies
  • Description
    Rabbit monoclonal [EPR1452(N)] to MEF2C
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human MEF2C aa 400 to the C-terminus. The exact sequence is proprietary.
    Database link: Q06413

  • Positive control
    • Saos-2, Raji, Human fetal brain and Rat kidney lysates; Human tonsil, Human spleen and Rat spleen tissues.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab191428 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/500 - 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/1000 - 1/2000. Detects a band of approximately 50-60 kDa (predicted molecular weight: 51 kDa).

Target

  • Function
    Transcription activator which binds specifically to the MEF2 element present in the regulatory regions of many muscle-specific genes. Controls cardiac morphogenesis and myogenesis, and is also involved in vascular development. Plays an essential role in hippocampal-dependent learning and memory by suppressing the number of excitatory synapses and thus regulating basal and evoked synaptic transmission. Crucial for normal neuronal development, distribution, and electrical activity in the neocortex. Necessary for proper development of megakaryocytes and platelets and for bone marrow B lymphopoiesis. Required for B-cell survival and proliferation in response to BCR stimulation, efficient IgG1 antibody responses to T-cell-dependent antigens and for normal induction of germinal center B cells. May also be involved in neurogenesis and in the development of cortical architecture (By similarity). Isoform 3 and isoform 4, which lack the repressor domain, are more active than isoform 1 and isoform 2.
  • Tissue specificity
    Expressed in brain and skeletal muscle.
  • Involvement in disease
    Defects in MEF2C are the cause of mental retardation-stereotypic movements-epilepsy and/or cerebral malformations (MRSME) [MIM:613443]. It is a disorder characterized by severe mental retardation, absent speech, hypotonia, poor eye contact and stereotypic movements. Dysmorphic features include high broad forehead with variable small chin, short nose with anteverted nares, large open mouth, upslanted palpebral fissures and prominent eyebrows. Some patients have seizures.
  • Sequence similarities
    Belongs to the MEF2 family.
    Contains 1 MADS-box domain.
    Contains 1 Mef2-type DNA-binding domain.
  • Developmental stage
    Expression is highest during the early stages of postnatal development, at later stages levels greatly decrease.
  • Domain
    The beta domain, missing in a number of isoforms, is required for enhancement of transcriptional activity.
  • Post-translational
    modifications
    Phosphorylation on Ser-59 enhances DNA binding activity (By similarity). Phosphorylation on Ser-396 is required for Lys-391 sumoylation and inhibits transcriptional activity.
    Acetylated by p300 on several sites in diffentiating myocytes. Acetylation on Lys-4 increases DNA binding and transactivation.
    Sumoylated on Lys-391 by SUMO2 but not by SUMO1 represses transcriptional activity.
    Proteolytically cleaved in cerebellar granule neurons, probably by caspase 7, following neurotoxicity. Preferentially cleaves the CDK5-mediated hyperphosphorylated form which leads to neuron apoptosis and transcriptional inactivation.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • C5DELq14.3 antibody
    • DEL5q14.3 antibody
    • MADS box transcription enhancer factor 2 polypeptide C (myocyte enhancer factor 2C) antibody
    • MADS box transcription enhancer factor 2, polypeptide C antibody
    • MEF2C antibody
    • MEF2C_HUMAN antibody
    • Myocyte enhancer factor 2C antibody
    • Myocyte specific enhancer factor 2C antibody
    • Myocyte-specific enhancer factor 2C antibody
    • OTTHUMP00000222409 antibody
    • Similar to MADS box transcription enhancer factor 2 polypeptide C antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling MEF2C with ab191428 at 1/1000 (0.5 μg/ml) with prediluted ImmunoHistoprobe(Ready to use) HRP Polymer for Rabbit IgG as secondary antibody. Negative control: Using PBS instead of primary ab, Secondary ab:ImmunoHistoprobe(Ready to use) HRP Polymer for Rabbit/Mouse IgG.

  • All lanes : Anti-MEF2C [EPR1452(N)] antibody (ab191428) at 1/1000 dilution

    Lane 1 : Saos-2 lysate
    Lane 2 : Raji lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 51 kDa
    Additional bands at: 50-60 kDa. We are unsure as to the identity of these extra bands.

  • Anti-MEF2C [EPR1452(N)] antibody (ab191428) at 1/1000 dilution + Human fetal brain lysate at 20 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 51 kDa
    Additional bands at: 50-60 kDa. We are unsure as to the identity of these extra bands.

  • Anti-MEF2C [EPR1452(N)] antibody (ab191428) at 1/1000 dilution + Rat brain lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 51 kDa
    Additional bands at: 50-60 kDa. We are unsure as to the identity of these extra bands.

  • Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labeling MEF2C with ab191428 at 1/1000 (0.5 μg/ml) with prediluted ImmunoHistoprobe(Ready to use) HRP Polymer for Rabbit IgG as secondary antibody. Negative control: Using PBS instead of primary ab, Secondary ab: ImmunoHistoprobe(Ready to use) HRP Polymer for Rabbit/Mouse IgG.

  • Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling MEF2C with ab191428 at 1/1000 (0.5 μg/ml) with prediluted ImmunoHistoprobe(Ready to use) HRP Polymer for Rabbit IgG as secondary antibody. Negative control: Using PBS instead of primary ab, Secondary ab: ImmunoHistoprobe(Ready to use) HRP Polymer for Rabbit/Mouse IgG.

References

This product has been referenced in:
  • Imasawa T  et al. High glucose repatterns human podocyte energy metabolism during differentiation and diabetic nephropathy. FASEB J 31:294-307 (2017). IHC ; Human . Read more (PubMed: 27825100) »
  • Xu Z  et al. MiR-135b-5p and MiR-499a-3p Promote Cell Proliferation and Migration in Atherosclerosis by Directly Targeting MEF2C. Sci Rep 5:12276 (2015). IHC-P . Read more (PubMed: 26184978) »

See all 2 Publications for this product

Customer reviews and Q&As

Application
Western blot
Sample
Monkey Cell lysate - whole cell (Kidney)
Gel Running Conditions
Non-reduced Non-Denaturing (Native)
Loading amount
30 µg
Specification
Kidney
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Aug 24 2016

Application
Western blot
Sample
Mouse Tissue lysate - whole (Brain)
Gel Running Conditions
Non-reduced Non-Denaturing (Native)
Loading amount
30 µg
Specification
Brain
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Aug 24 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Cow Cell lysate - whole cell (Lung)
Gel Running Conditions
Non-reduced Non-Denaturing (Native)
Loading amount
30 µg
Specification
Lung
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Aug 24 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (Brain)
Permeabilization
Yes - 0.3% Trotpn X-100
Specification
Brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Aug 10 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Kidney)
Gel Running Conditions
Non-reduced Non-Denaturing (Native)
Loading amount
30 µg
Specification
Kidney
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Aug 10 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Brain)
Permeabilization
Yes - 0.3% Trotpn X-100
Specification
Brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Jul 26 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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