Anti-MEF2C (phospho S387) antibody (ab111576)

Overview

  • Product nameAnti-MEF2C (phospho S387) antibody
    See all MEF2C primary antibodies
  • Description
    Rabbit polyclonal to MEF2C (phospho S387)
  • Specificityab111576 detects endogenous levels of MEF2C only when phosphorylated at serine 387 (Human: Ser387; Mouse: Ser385).
  • Tested applicationsSuitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic phosphopeptide derived from Human MEF2C around the phosphorylation site of Serine 387 (T-Q-SP-L-N).

  • Positive control
    • Human heart tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab111576 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionTranscription activator which binds specifically to the MEF2 element present in the regulatory regions of many muscle-specific genes. Controls cardiac morphogenesis and myogenesis, and is also involved in vascular development. Plays an essential role in hippocampal-dependent learning and memory by suppressing the number of excitatory synapses and thus regulating basal and evoked synaptic transmission. Crucial for normal neuronal development, distribution, and electrical activity in the neocortex. Necessary for proper development of megakaryocytes and platelets and for bone marrow B lymphopoiesis. Required for B-cell survival and proliferation in response to BCR stimulation, efficient IgG1 antibody responses to T-cell-dependent antigens and for normal induction of germinal center B cells. May also be involved in neurogenesis and in the development of cortical architecture (By similarity). Isoform 3 and isoform 4, which lack the repressor domain, are more active than isoform 1 and isoform 2.
  • Tissue specificityExpressed in brain and skeletal muscle.
  • Involvement in diseaseDefects in MEF2C are the cause of mental retardation-stereotypic movements-epilepsy and/or cerebral malformations (MRSME) [MIM:613443]. It is a disorder characterized by severe mental retardation, absent speech, hypotonia, poor eye contact and stereotypic movements. Dysmorphic features include high broad forehead with variable small chin, short nose with anteverted nares, large open mouth, upslanted palpebral fissures and prominent eyebrows. Some patients have seizures.
  • Sequence similaritiesBelongs to the MEF2 family.
    Contains 1 MADS-box domain.
    Contains 1 Mef2-type DNA-binding domain.
  • Developmental stageExpression is highest during the early stages of postnatal development, at later stages levels greatly decrease.
  • DomainThe beta domain, missing in a number of isoforms, is required for enhancement of transcriptional activity.
  • Post-translational
    modifications
    Phosphorylation on Ser-59 enhances DNA binding activity (By similarity). Phosphorylation on Ser-396 is required for Lys-391 sumoylation and inhibits transcriptional activity.
    Acetylated by p300 on several sites in diffentiating myocytes. Acetylation on Lys-4 increases DNA binding and transactivation.
    Sumoylated on Lys-391 by SUMO2 but not by SUMO1 represses transcriptional activity.
    Proteolytically cleaved in cerebellar granule neurons, probably by caspase 7, following neurotoxicity. Preferentially cleaves the CDK5-mediated hyperphosphorylated form which leads to neuron apoptosis and transcriptional inactivation.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • C5DELq14.3 antibody
    • DEL5q14.3 antibody
    • MADS box transcription enhancer factor 2 polypeptide C (myocyte enhancer factor 2C) antibody
    • MADS box transcription enhancer factor 2, polypeptide C antibody
    • MEF2C antibody
    • MEF2C_HUMAN antibody
    • Myocyte enhancer factor 2C antibody
    • Myocyte specific enhancer factor 2C antibody
    • Myocyte-specific enhancer factor 2C antibody
    • OTTHUMP00000222409 antibody
    • Similar to MADS box transcription enhancer factor 2 polypeptide C antibody
    see all

Anti-MEF2C (phospho S387) antibody images

  • ab111576, at 1/50 dilution, staining MEF2C in paraffin-embedded Human heart tissue by Immunohistochemistry, in the presence (right panel) or absence (left panel) of immunising peptide.

References for Anti-MEF2C (phospho S387) antibody (ab111576)

ab111576 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (C2C12)
Loading amount 40 µg
Specification C2C12
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Abcam user community

Verified customer

Submitted Dec 07 2012

Thank you again for testing ab111576 with mouse samples.

My colleague has contacted me and notified me of the trouble using thiscode yesterday. I apologize forthe confusion and for the inconvenience. The original purchase of ab111576 needs to...

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Thank you very much for your reply and for the information.

DISCOUNT CODE:***
Expiration date: December 24, 2012

This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit a...

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Thank you very much for your reply and for testing this antibody.

I'd just like to confirm whether you will be testing the antibody with rat or mouse samples? C2C12 is a mouse myoblast cell line-

http://www.atcc.org/ATCCAdvancedCata...

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Thank you very much for contacting us with your question.

At this time, we have not tested ab111576 with rat samples so the cross-reactivity is unknown. I tried to run an alignment with the immunogen region against the rat protein, however I ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"