Overview

  • Product nameAnti-MEF2C (phospho S396) antibody
    See all MEF2C primary antibodies
  • Description
    Rabbit polyclonal to MEF2C (phospho S396)
  • SpecificityDetects endogenous levels of MEF2C only when phosphorylated at serine 396.
  • Tested applicationsSuitable for: WB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic phosphopeptide derived from Human MEF2C around the phosphorylation site of serine 396 (P-V-SP-P-P)

  • Positive control

Properties

Applications

Our Abpromise guarantee covers the use of ab78888 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 51 kDa.
IHC-P 1/50 - 1/100.
ELISA 1/5000.

Target

  • FunctionTranscription activator which binds specifically to the MEF2 element present in the regulatory regions of many muscle-specific genes. Controls cardiac morphogenesis and myogenesis, and is also involved in vascular development. Plays an essential role in hippocampal-dependent learning and memory by suppressing the number of excitatory synapses and thus regulating basal and evoked synaptic transmission. Crucial for normal neuronal development, distribution, and electrical activity in the neocortex. Necessary for proper development of megakaryocytes and platelets and for bone marrow B lymphopoiesis. Required for B-cell survival and proliferation in response to BCR stimulation, efficient IgG1 antibody responses to T-cell-dependent antigens and for normal induction of germinal center B cells. May also be involved in neurogenesis and in the development of cortical architecture (By similarity). Isoform 3 and isoform 4, which lack the repressor domain, are more active than isoform 1 and isoform 2.
  • Tissue specificityExpressed in brain and skeletal muscle.
  • Involvement in diseaseDefects in MEF2C are the cause of mental retardation-stereotypic movements-epilepsy and/or cerebral malformations (MRSME) [MIM:613443]. It is a disorder characterized by severe mental retardation, absent speech, hypotonia, poor eye contact and stereotypic movements. Dysmorphic features include high broad forehead with variable small chin, short nose with anteverted nares, large open mouth, upslanted palpebral fissures and prominent eyebrows. Some patients have seizures.
  • Sequence similaritiesBelongs to the MEF2 family.
    Contains 1 MADS-box domain.
    Contains 1 Mef2-type DNA-binding domain.
  • Developmental stageExpression is highest during the early stages of postnatal development, at later stages levels greatly decrease.
  • DomainThe beta domain, missing in a number of isoforms, is required for enhancement of transcriptional activity.
  • Post-translational
    modifications
    Phosphorylation on Ser-59 enhances DNA binding activity (By similarity). Phosphorylation on Ser-396 is required for Lys-391 sumoylation and inhibits transcriptional activity.
    Acetylated by p300 on several sites in diffentiating myocytes. Acetylation on Lys-4 increases DNA binding and transactivation.
    Sumoylated on Lys-391 by SUMO2 but not by SUMO1 represses transcriptional activity.
    Proteolytically cleaved in cerebellar granule neurons, probably by caspase 7, following neurotoxicity. Preferentially cleaves the CDK5-mediated hyperphosphorylated form which leads to neuron apoptosis and transcriptional inactivation.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • C5DELq14.3 antibody
    • DEL5q14.3 antibody
    • MADS box transcription enhancer factor 2 polypeptide C (myocyte enhancer factor 2C) antibody
    • MADS box transcription enhancer factor 2, polypeptide C antibody
    • MEF2C antibody
    • MEF2C_HUMAN antibody
    • Myocyte enhancer factor 2C antibody
    • Myocyte specific enhancer factor 2C antibody
    • Myocyte-specific enhancer factor 2C antibody
    • OTTHUMP00000222409 antibody
    • Similar to MADS box transcription enhancer factor 2 polypeptide C antibody
    see all

Anti-MEF2C (phospho S396) antibody images

  • Immunohistochemistry analysis of paraffin-embedded Human brain tissue using ab78888 at 1/50 dilution in the absence or presence of the immunzing phosphopeptide.
  • All lanes : Anti-MEF2C (phospho S396) antibody (ab78888) at 1/500 dilution

    Lane 1 : Extracts from 24 hours starved 3T3 cells
    Lane 2 : Extracts from 24 hours starved 3T3 cells with immunizing phosphopeptide at 5 µg

    Lysates/proteins at 20 µg per lane.


    Predicted band size : 51 kDa
    Observed band size : 51 kDa

References for Anti-MEF2C (phospho S396) antibody (ab78888)

This product has been referenced in:
  • Chen B  et al. Inhibition of miR-29c promotes proliferation, and inhibits apoptosis and differentiation in P19 embryonic carcinoma cells. Mol Med Rep 13:2527-35 (2016). WB ; Mouse . Read more (PubMed: 26848028) »

See 1 Publication for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (neuroblastoma)
Permeabilization Yes - 0.1% Troton X-100
Specification neuroblastoma
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Fixative Paraformaldehyde
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Submitted Sep 30 2016

Application Immunocytochemistry/ Immunofluorescence
Sample Monkey Cell (Kidney)
Permeabilization Yes - 0.1% Troton X-100
Specification Kidney
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Fixative Paraformaldehyde
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Submitted Sep 30 2016

Application Immunohistochemistry (Frozen sections)
Sample Human Tissue sections (Brain)
Permeabilization Yes - 0.3% Triton X-100
Specification Brain
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Fixative Paraformaldehyde
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Submitted Jul 20 2016

Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Brain)
Permeabilization Yes - 0.3% Triton X-100
Specification Brain
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Fixative Paraformaldehyde
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Submitted Jul 20 2016

Application Western blot
Sample Cow Cell lysate - whole cell (Lung)
Gel Running Conditions Non-reduced Non-Denaturing (Native)
Loading amount 30 µg
Specification Lung
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
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Submitted Jul 14 2016

Application Western blot
Sample Mouse Tissue lysate - whole (Brain)
Gel Running Conditions Non-reduced Non-Denaturing (Native)
Loading amount 30 µg
Specification Brain
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
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Submitted Jul 11 2016

Application Western blot
Sample Monkey Cell lysate - whole cell (Kidney)
Gel Running Conditions Non-reduced Non-Denaturing (Native)
Loading amount 30 µg
Specification Kidney
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
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Submitted Jul 11 2016

Application Western blot
Sample Human Cell lysate - whole cell (Kidney)
Gel Running Conditions Non-reduced Non-Denaturing (Native)
Loading amount 30 µg
Specification Kidney
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
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Submitted Jul 11 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"