Overview

  • Product name
  • Description
    Rabbit polyclonal to MEF2D
  • Tested applications
    Suitable for: WB, IP, EMSA, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human, Spermophilus tridecemlineatus
  • Immunogen

    Recombinant fragment, corresponding to amino acids 292-514 of Human MEF2D

Properties

Applications

Our Abpromise guarantee covers the use of ab32845 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).
IP Use at an assay dependent concentration. Use 4µl for 1ml cell lysate.
EMSA Use at an assay dependent concentration.
IHC-P Use a concentration of 2 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • Function
    Transcriptional activator which binds specifically to the MEF2 element, 5'-YTA[AT](4)TAR-3', found in numerous muscle-specific, growth factor- and stress-induced genes. Mediates cellular functions not only in skeletal and cardiac muscle development, but also in neuronal differentiation and survival. Plays diverse roles in the control of cell growth, survival and apoptosis via p38 MAPK signaling in muscle-specific and/or growth factor-related transcription. Plays a critical role in the regulation of neuronal apoptosis.
  • Sequence similarities
    Belongs to the MEF2 family.
    Contains 1 MADS-box domain.
    Contains 1 Mef2-type DNA-binding domain.
  • Developmental stage
    Present in myotubes and also in undifferentiated myoblasts.
  • Domain
    The beta domain, missing in a number of isoforms, is required for enhancement of transcriptional activity.
  • Post-translational
    modifications
    Phosphorylated on Ser-444 by CDK5 is required for Lys-439 sumoylation and inhibits transcriptional activity. In neurons, enhanced CDK5 activity induced by neurotoxins promotes caspase 3-mediated cleavage leading to neuron apoptosis. Phsophorylation on Ser-180 can be enhanced by EGF.
    Acetylated on Lys-439 by CREBBP. Deacetylated by SIRT1.
    Sumoylated on Lys-439 by SUMO2 but not SUMO1; which inhibits transcriptional activity and myogenic activity. Desumoylated by SENP3.
    Proteolytically cleaved in cerebellar granule neurons on several sites by caspase 7 following neurotoxicity. Preferentially cleaves the CDK5-mediated hyperphosphorylated form which leads to neuron apoptosis and transcriptional inactivation.
  • Cellular localization
    Nucleus. Translocated by HDAC4 to nuclear dots.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZp686I1536 antibody
    • MADS box transcription factor 2 polypeptide D antibody
    • Mef2d antibody
    • MEF2D_HUMAN antibody
    • Myocyte enhancer factor 2D antibody
    • Myocyte specific enhancer factor 2, polypeptide D antibody
    • Myocyte specific enhancer factor 2D antibody
    • Myocyte-specific enhancer factor 2D antibody
    see all

Images

  • Immunoprecipitation of 35S-methionine labelled HeLa cells with anti-MEF2D sera. 1: pre-immune sera; 2: ab32845 anti-MEF2D sera.
  • ab32845 (2µg/ml) staining MEF2D in human skin using an automated system (DAKO Autostainer Plus). Using this protocol there is strong nuclear staining.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate pH6.1 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

References

This product has been referenced in:
  • Pon JR  et al. MEF2B mutations in non-Hodgkin lymphoma dysregulate cell migration by decreasing MEF2B target gene activation. Nat Commun 6:7953 (2015). WB ; Human . Read more (PubMed: 26245647) »
  • Pearen MA  et al. The nuclear receptor, Nor-1, markedly increases type II oxidative muscle fibers and resistance to fatigue. Mol Endocrinol 26:372-84 (2012). WB ; Mouse . Read more (PubMed: 22282471) »

See all 6 Publications for this product

Customer reviews and Q&As

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.
I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has c...

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Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (placenta)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate pH6 100C 20 min
Permeabilization
No
Specification
placenta
Blocking step
H2O2 as blocking agent for 10 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted Nov 13 2015

Thank you for your reply.
Mouse is actually also untested for ab59200 - it is listed as predicted to react based on sequence homology. Thus, we would not guarantee it for this species. But, please feel free to submit another Abreview for mouse sam...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Spermophilus tridecemlineatus Tissue lysate - whole (Liver, Heart, Skeletal muscle)
Loading amount
20 µg
Specification
Liver, Heart, Skeletal muscle
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
BSA as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Mr. James Bjork

Verified customer

Submitted Oct 26 2012

Thank you for contacting us. As you can see on our website the image we have is at 68kD. The predicted moleclular weight is based on amino acid sequence only, while this protein has many post-translational modifications which may increase the MW. This,...

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Thank you very much for your email and for your interest in ab32845.

We have recently changed our testing program, and the new program is called AbTrial-

http://www.abcam.com/index.html?pageconfig=resource&rid=11998&source=p...

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