Anti-MEF2D antibody (ab32845)
Key features and details
- Rabbit polyclonal to MEF2D
- Suitable for: IP, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-MEF2D antibody
See all MEF2D primary antibodies -
Description
Rabbit polyclonal to MEF2D -
Host species
Rabbit -
Tested applications
Suitable for: IP, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment, corresponding to amino acids 292-514 of Human MEF2D
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: Whole serum -
Concentration information loading...
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Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab32845 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IP |
Use at an assay dependent concentration. Use 4µl for 1ml cell lysate.
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IHC-P | (1) |
Use a concentration of 2 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Notes |
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IP
Use at an assay dependent concentration. Use 4µl for 1ml cell lysate. |
IHC-P
Use a concentration of 2 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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Function
Transcriptional activator which binds specifically to the MEF2 element, 5'-YTA[AT](4)TAR-3', found in numerous muscle-specific, growth factor- and stress-induced genes. Mediates cellular functions not only in skeletal and cardiac muscle development, but also in neuronal differentiation and survival. Plays diverse roles in the control of cell growth, survival and apoptosis via p38 MAPK signaling in muscle-specific and/or growth factor-related transcription. Plays a critical role in the regulation of neuronal apoptosis. -
Sequence similarities
Belongs to the MEF2 family.
Contains 1 MADS-box domain.
Contains 1 Mef2-type DNA-binding domain. -
Developmental stage
Present in myotubes and also in undifferentiated myoblasts. -
Domain
The beta domain, missing in a number of isoforms, is required for enhancement of transcriptional activity. -
Post-translational
modificationsPhosphorylated on Ser-444 by CDK5 is required for Lys-439 sumoylation and inhibits transcriptional activity. In neurons, enhanced CDK5 activity induced by neurotoxins promotes caspase 3-mediated cleavage leading to neuron apoptosis. Phsophorylation on Ser-180 can be enhanced by EGF.
Acetylated on Lys-439 by CREBBP. Deacetylated by SIRT1.
Sumoylated on Lys-439 by SUMO2 but not SUMO1; which inhibits transcriptional activity and myogenic activity. Desumoylated by SENP3.
Proteolytically cleaved in cerebellar granule neurons on several sites by caspase 7 following neurotoxicity. Preferentially cleaves the CDK5-mediated hyperphosphorylated form which leads to neuron apoptosis and transcriptional inactivation. -
Cellular localization
Nucleus. Translocated by HDAC4 to nuclear dots. - Information by UniProt
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Database links
- Entrez Gene: 4209 Human
- Omim: 600663 Human
- SwissProt: Q14814 Human
- Unigene: 314327 Human
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Alternative names
- DKFZp686I1536 antibody
- MADS box transcription factor 2 polypeptide D antibody
- Mef2d antibody
see all
Images
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Immunoprecipitation of 35S-methionine labelled HeLa cells with anti-MEF2D sera. 1: pre-immune sera; 2: ab32845 anti-MEF2D sera.
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ab32845 (2µg/ml) staining MEF2D in human skin using an automated system (DAKO Autostainer Plus). Using this protocol there is strong nuclear staining.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate pH6.1 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
Protocols
Datasheets and documents
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Datasheet download
References (15)
ab32845 has been referenced in 15 publications.
- Pingul BY et al. Dissection of the MEF2D-IRF8 transcriptional circuit dependency in acute myeloid leukemia. iScience 25:105139 (2022). PubMed: 36193052
- Cilenti F et al. A PGE2-MEF2A axis enables context-dependent control of inflammatory gene expression. Immunity 54:1665-1682.e14 (2021). PubMed: 34129840
- Ling B et al. Nucleolar and spindle-associated protein 1 promotes non-small cell lung cancer progression and serves as an effector of myocyte enhancer factor 2D. Oncol Rep 45:1044-1058 (2021). PubMed: 33650655
- Huang L et al. Protocatechuic acid attenuates ß-secretase activity and okadaic acid-induced autophagy via the Akt/GSK-3ß/MEF2D pathway in PC12 cells. Mol Med Rep 21:1328-1335 (2020). PubMed: 31894327
- Cheng Y et al. NeuroD1 Dictates Tumor Cell Differentiation in Medulloblastoma. Cell Rep 31:107782 (2020). PubMed: 32579914