Anti-MEF2D (phospho S444) antibody (ab59200)

Overview

  • Product name
    Anti-MEF2D (phospho S444) antibody
    See all MEF2D primary antibodies
  • Description
    Rabbit polyclonal to MEF2D (phospho S444)
  • Specificity
    Detects endogenous levels of MEF2D only when phosphorylated at serine 444.
  • Tested applications
    Suitable for: IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic phosphopeptide (Human) from around the phosphorylation site of serine 444 (PVSPPS).

  • Positive control
    • Human lung carcinoma tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab59200 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
ELISA 1/10000.

Target

  • Function
    Transcriptional activator which binds specifically to the MEF2 element, 5'-YTA[AT](4)TAR-3', found in numerous muscle-specific, growth factor- and stress-induced genes. Mediates cellular functions not only in skeletal and cardiac muscle development, but also in neuronal differentiation and survival. Plays diverse roles in the control of cell growth, survival and apoptosis via p38 MAPK signaling in muscle-specific and/or growth factor-related transcription. Plays a critical role in the regulation of neuronal apoptosis.
  • Sequence similarities
    Belongs to the MEF2 family.
    Contains 1 MADS-box domain.
    Contains 1 Mef2-type DNA-binding domain.
  • Developmental stage
    Present in myotubes and also in undifferentiated myoblasts.
  • Domain
    The beta domain, missing in a number of isoforms, is required for enhancement of transcriptional activity.
  • Post-translational
    modifications
    Phosphorylated on Ser-444 by CDK5 is required for Lys-439 sumoylation and inhibits transcriptional activity. In neurons, enhanced CDK5 activity induced by neurotoxins promotes caspase 3-mediated cleavage leading to neuron apoptosis. Phsophorylation on Ser-180 can be enhanced by EGF.
    Acetylated on Lys-439 by CREBBP. Deacetylated by SIRT1.
    Sumoylated on Lys-439 by SUMO2 but not SUMO1; which inhibits transcriptional activity and myogenic activity. Desumoylated by SENP3.
    Proteolytically cleaved in cerebellar granule neurons on several sites by caspase 7 following neurotoxicity. Preferentially cleaves the CDK5-mediated hyperphosphorylated form which leads to neuron apoptosis and transcriptional inactivation.
  • Cellular localization
    Nucleus. Translocated by HDAC4 to nuclear dots.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZp686I1536 antibody
    • MADS box transcription factor 2 polypeptide D antibody
    • Mef2d antibody
    • MEF2D_HUMAN antibody
    • Myocyte enhancer factor 2D antibody
    • Myocyte specific enhancer factor 2, polypeptide D antibody
    • Myocyte specific enhancer factor 2D antibody
    • Myocyte-specific enhancer factor 2D antibody
    see all

Images

  • Immunohistochemical analysis of paraffin embedded human lung carcinoma tissue using ab59200 at 1/50 dilution. Samples were untreated (left) or treated with immunizing peptide (right).

References

ab59200 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Thank you for your reply.
Mouse is actually also untested for ab59200 - it is listed as predicted to react based on sequence homology. Thus, we would not guarantee it for this species. But, please feel free to submit another Abreview for mouse sam...

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Thank you for submitting an Abreview for ab59200. As you may have noticed, your review has now been published on our website.
Since you obtained poor results using the antibody in an untested species and application, we would like to follow up on t...

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Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Spermophilus tridecemlineatus Tissue lysate - whole (Heart)
Loading amount
40 µg
Specification
Heart
Gel Running Conditions
Reduced Denaturing (10% Tris-Glycine)
Blocking step
BSA as blocking agent for 15 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Mr. James Bjork

Verified customer

Submitted Nov 06 2012

Thank you very much for contacting us with your enquiry. I've created the discount code below-
DISCOUNT CODE: ***
Expiration date: January 28, 2013
This code will give you 1 free primary antibody before the expiration date. To redeem this...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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