Our Abpromise guarantee covers the use of ab108446 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||Use at an assay dependent concentration.|
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 111 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
ab108446 staining MEGF11 in zebrafish retina (muller glia cells) tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, permeabilized with Triton X-100 and blocked with 5% BSA for 1 hour at 23°C. Tris-HCl was used for antigen retrieval. Samples were incubated with primary antibody (1/100) for 16 hours at 4°C. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody.
ab108446 has not yet been referenced specifically in any publications.