Overview

  • Product nameAnti-MEGF11 antibody
    See all MEGF11 primary antibodies
  • Description
    Rabbit polyclonal to MEGF11
  • Tested applicationsSuitable for: IHC-Fr, WBmore details
  • Species reactivity
    Reacts with: Human, Zebrafish
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 827-876 (LMMEELNPYT KISPALGAER HSVGAVTGIM LLLFLIVVLL GLFAWHRRRQ) of Human MEGF11 (NP_115821)

  • Positive control
    • Human fetal muscle lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab108446 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 111 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

Anti-MEGF11 antibody images

  • ab108446 staining MEGF11 in zebrafish retina (muller glia cells) tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, permeabilized with Triton X-100 and blocked with 5% BSA for 1 hour at 23°C. Tris-HCl was used for antigen retrieval. Samples were incubated with primary antibody (1/100) for 16 hours at 4°C. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody.

    See Abreview

  • Anti-MEGF11 antibody (ab108446) at 1 µg/ml + Human fetal muscle lysate at 10 µg

    Predicted band size : 111 kDa
    Gel concentration: 12%

References for Anti-MEGF11 antibody (ab108446)

ab108446 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Frozen sections)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Sample Zebrafish Tissue sections (Retina, Muller glia cells)
Specification Retina, Muller glia cells
Permeabilization Yes - triton X
Fixative Paraformaldehyde
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Submitted Oct 02 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"