Recombinant
RabMAb

Anti-Meis homeobox 3 antibody [EPR15416] (ab185961)

Overview

  • Product name
    Anti-Meis homeobox 3 antibody [EPR15416]
  • Description
    Rabbit monoclonal [EPR15416] to Meis homeobox 3
  • Tested applications
    Suitable for: WB, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Meis homeobox 3 aa 1-100. The exact sequence is proprietary.
    Database link: Q99687

  • Positive control
    • MCF7, HepG2, Human fetal lung and heart, rat brain, rat heart, rat kidney, rat spleen, RAW 264.7 and NIH 3T3 cell lysates; HeLa cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab185961 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa).
Flow Cyt 1/210. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Target

Images

  • All lanes : Anti-Meis homeobox 3 antibody [EPR15416] (ab185961) at 1/5000 dilution

    Lane 1 : MCF7 cell lysate
    Lane 2 : HepG2 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size : 41 kDa
  • All lanes : Anti-Meis homeobox 3 antibody [EPR15416] (ab185961) at 1/5000 dilution

    Lane 1 : Human fetal lung lysate
    Lane 2 : Human fetal heart lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size : 41 kDa
  • All lanes : Anti-Meis homeobox 3 antibody [EPR15416] (ab185961) at 1/1000 dilution

    Lane 1 : Rat brain lysate
    Lane 2 : Rat heart lysate
    Lane 3 : Rat kidney lysate
    Lane 4 : Rat spleen lysate
    Lane 5 : RAW 264.7 cell lysate
    Lane 6 : NIH 3T3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size : 41 kDa
  • Flow Cytometric analysis of 2% paraformaldehyde-fixed HeLa cells labeling Meis homeobox 3 with ab185961 at 1/210 dilution (red) compared with a rabbit monoclonal IgG isotype control (green). Detection utilised Goat anti rabbit IgG (FITC) at 1/150 dilution.

References

ab185961 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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