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Products:Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Developmental Families >> HOX
Overview
Product name
Anti-MEIS1 antibody - ChIP Grade
See all MEIS1 products (3) ...
Description
Rabbit polyclonal to MEIS1 - ChIP Grade
Tested applications
IHC-P, ChIP, WBmore details
Cross reactivity
Reacts with
Mouse, Human, Zebrafish
Predicted to work with
Dog
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 200 - 300 of Human MEIS1.
(Peptide available as ab22467.)
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Applications
Show applications key
Our Abpromise guarantee covers the use of ab19867 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: Use a concentration of 1 µg/mlPerform heat mediated antigen retrieval before commencing with IHC staining protocol.
ChIP: Use at an assay dependent dilution. (PubMed: 20971928)
WB: Use a concentration of 1 µg/mlDetects a band of approximately 43 kDa (predicted molecular weight: 37 kDa).
Target
Function
Acts as a transcriptional regulator of PAX6. Acts as a transcriptional activator of PF4 in complex with PBX1 or PBX2. Required for hematopoiesis, megakaryocyte lineage development and vascular patterning. May function as a cofactor for HOXA7 and HOXA9 in the induction of myeloid leukemias.
Tissue specificity
Expressed at low level in normal immunohepatopoietic tissues, including the fetal liver. Expressed in a subset of myeloid leukemia cell lines, with the highest expression seen in those with a megakaryocytic-erythroid phenotype. Also expressed at high levels in the cerebellum.
Involvement in disease
Defects in MEIS1 could be a cause of susceptibility to restless legs syndrome type 7 (RLS7) [MIM:612853]. Restless legs syndrome (RLS) is a neurologic sleep/wake disorder characterized by uncomfortable and unpleasant sensations in the legs that appear at rest, usually at night, inducing an irresistible desire to move the legs. The disorder results in nocturnal insomnia and chronic sleep deprivation.
Sequence similarities
Belongs to the TALE/MEIS homeobox family.
Contains 1 homeobox DNA-binding domain.
Cellular localization
Nucleus.
Target information above from: UniProt accessionO00470
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Meis1 myeloid ecotropic viral integration site 1 homolog mouse antibody
- Homeo box protein Meis1 antibody
- Homeobox protein Meis1 antibody
see all
Database links
- Entrez Gene: 4211 Human
- Entrez Gene: 17268 Mouse
- Entrez Gene: 170446 Zebrafish
- Omim: 601739 Human
- SwissProt: O00470 Human
- SwissProt: Q60954 Mouse
- SwissProt: Q90XH2 Zebrafish
- Unigene: 526754 Human
see all
Anti-MEIS1 antibody - ChIP Grade images:
Western blot - MEIS1 antibody (ab19867)
Anti-MEIS1 antibody - ChIP Grade (ab19867) at 1 µg/ml + HEK293 Wcl Transiently Overexpressing MEIS1 at 5 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) (ab65484) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 37 kDa
Observed band size : 45 kDa (why is the actual band size different from the predicted?)
Western blot - MEIS1 antibody (ab19867)
All lanes : Anti-MEIS1 antibody - ChIP Grade (ab19867) at 1 µg/ml
Lane 1 : KG-1 (Human myeloid cell line) Nuclear Lysate (ab14854)
Lane 2 : KG-1 (Human myeloid cell line) Nuclear Lysate (ab14854) with MEIS1 peptide (ab22467) at 1 µg/ml
Lysates/proteins at 20 µg per lane.
Predicted band size : 37 kDa
Observed band size : 37 kDa
This antibody detects a band at approximately 37kDa which is smaller than the expected size of 46kDa for MEIS1. We cannot explain the difference in the size of the expected band. This band is blocked by the addition of the immunizing peptide, suggesting that it is a specific interaction.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MEIS1 antibody (ab19867)
IHC image of MEIS1 staining in human tonsil FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab19867, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
References for Anti-MEIS1 antibody - ChIP Grade (ab19867)
This product has been referenced in:
- Huang Yet al. Identification and characterization of Hoxa9 binding sites in hematopoietic cells. Blood 119:388-98 (2012).Read more (PubMed: 22072553) »
- Novershtern Net al. Densely interconnected transcriptional circuits control cell states in human hematopoiesis. Cell 144:296-309 (2011). ChIP; Human.Read more (PubMed: 21241896) »
See all 8 publications for this product
Publishing research using ab19867? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"