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Western blot - MEIS1 antibody (ab19867)
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Western blot - MEIS1 antibody (ab19867)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MEIS1 antibody (ab19867)
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Product Name
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MEIS1 antibody
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See all MEIS1 antibodies (2)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to MEIS1
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Immunogen
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Synthetic peptide conjugated to KLH derived from within residues 200 - 300 of Human MEIS1.
(Peptide available as ab22467.)
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Reacts with
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Hu, Ms
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Predicted to react
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Dog
(Due to sequence homology)
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Tested applications
(see key)
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IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
IHC-P: Use at a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 43 kDa (predicted molecular weight: 37 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Nuclear
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Research areas
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Stem Cells >> Hematopoietic Progenitors >> Myeloid >> Myeloid Progenitor
Chromatin and Nuclear Signaling >> Transcription >> Domain Families >> Developmental Families >> HOX
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Relevance
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MEIS1 is a member of the TALE (three amino acid loop extension) family of homeodomain containing proteins. In addition to binding with other Hox proteins, MEIS1 also forms heterodimers with the ubiquitously expressed Pbx proteins, including Pbx1, Pbx2 and Pbx3, and these complexes contain distinct DNA binding specificities. MEIS1 is implicated in oncogenesis as it is overexpressed as a result of adjacent retroviral insertion in BHX2 myeloid leukemias. It plays an important role in megakaryocytic gene expression.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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Batch dependent within range: 0.10 - 0.34 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this MEIS1 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for MEIS1 antibody images, references, products related to ab19867 and other tools.
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MEIS1 antibody images:
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 Western blot - MEIS1 antibody (ab19867)
MEIS1 antibody (ab19867) at 1 µg/ml + HEK293 Wcl Transiently Overexpressing MEIS1 at 5 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) (ab65484) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 37 kDa
Observed band size : 45 kDa (why is the actual band size different from the predicted?)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MEIS1 antibody (ab19867)
IHC image of MEIS1 staining in human tonsil FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab19867, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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References for MEIS1 antibody (ab19867)
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This product has been used in:
Horman SR et al. Gfi1 integrates progenitor versus granulocytic transcriptional programming. Blood 113:5466-75 (2009). WB; Mouse. PubMed: 19346496
If you publish research using ab19867 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to MEIS1
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MEIS1 antibody - more information
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Customer reviews (feedback) regarding MEIS1 antibody |
Customer FAQs regarding MEIS1 antibody |
Protocols for MEIS1 antibody |
Price and availability of products related to MEIS1 antibody |
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MEIS1 antibody - related products:
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MEIS1 antibody - other tools:
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Contact Abcam with a Technical Enquiry about MEIS1 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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