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Products:Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Developmental Families >> HOX
Anti-MEIS1 antibody - ChIP Grade (ab19867)
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Proteins and Peptides
ab22467
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Secondary antibodies
Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »This product is covered by the Abpromise guarantee. Our scientific support team are available to answer any questions or queries - fill out an inquiry form for ab19867 for help.
There are no answered questions for ab19867
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Western blot - Anti-MEIS1 antibody - ChIP Grade (ab19867)
Anti-MEIS1 antibody - ChIP Grade (ab19867) at 1 µg/ml + KG-1 (Human myeloid cell line) Nuclear Lysate at 15 µg
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
Performed under reducing conditions.
Predicted band size : 43, 50 kDa
Observed band size : 40,48 kDa (why is the actual band size different from the predicted?)
Additional bands at : 58 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 12 minutes
This antibody was raised against an immunogen that is predicted to recognize isoforms 1 and 2 of MEIS1. The predicted molecular weights of isoforms 1 and 2 are 43 kDa and 50 kDa respectively.
Abcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
Western blot - MEIS1 antibody (ab19867)
Anti-MEIS1 antibody - ChIP Grade (ab19867) at 1 µg/ml + HEK293 Wcl Transiently Overexpressing MEIS1 at 5 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) (ab65484) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 43, 50 kDa
Observed band size : 45 kDa (why is the actual band size different from the predicted?)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MEIS1 antibody (ab19867)
IHC image of MEIS1 staining in human tonsil FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab19867, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
MEIS1 antibody - ChIP Grade for Western blot in Mouse (19867)
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