Overview

  • Product nameAnti-MEIS1 antibody - ChIP Grade
    See all MEIS1 primary antibodies
  • Description
    Rabbit polyclonal to MEIS1 - ChIP Grade
  • Tested applicationsSuitable for: IHC-P, CHIPseq, ChIP, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Zebrafish
    Predicted to work with: Dog
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 200 - 300 of Human MEIS1.

    (Peptide available as ab22467.)

  • Positive control
    • This antibody gave a positive signal in the following nuclear cell extract: KG-1. This antibody gave a positive signal in the following FFPE tissue: Human tonsil, and Rat normal brain.
  • General notesFor a detailed method for MEIS1 ChIP-seq in RA-treated P19.6 cells, please see the protocol described by Dr Aurelien Serandour at http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM819083.

Properties

Applications

Our Abpromise guarantee covers the use of ab19867 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
CHIPseq Use at an assay dependent concentration. PubMed: 22730288
ChIP Use at an assay dependent concentration. PubMed: 20971928
WB Use a concentration of 1 µg/ml. Detects a band of approximately 40, 48 kDa (predicted molecular weight: 43, 50 kDa). Abcam recommends using milk as the blocking agent.

Target

  • FunctionActs as a transcriptional regulator of PAX6. Acts as a transcriptional activator of PF4 in complex with PBX1 or PBX2. Required for hematopoiesis, megakaryocyte lineage development and vascular patterning. May function as a cofactor for HOXA7 and HOXA9 in the induction of myeloid leukemias.
  • Tissue specificityExpressed at low level in normal immunohepatopoietic tissues, including the fetal liver. Expressed in a subset of myeloid leukemia cell lines, with the highest expression seen in those with a megakaryocytic-erythroid phenotype. Also expressed at high levels in the cerebellum.
  • Involvement in diseaseDefects in MEIS1 could be a cause of susceptibility to restless legs syndrome type 7 (RLS7) [MIM:612853]. Restless legs syndrome (RLS) is a neurologic sleep/wake disorder characterized by uncomfortable and unpleasant sensations in the legs that appear at rest, usually at night, inducing an irresistible desire to move the legs. The disorder results in nocturnal insomnia and chronic sleep deprivation.
  • Sequence similaritiesBelongs to the TALE/MEIS homeobox family.
    Contains 1 homeobox DNA-binding domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Homeo box protein Meis1 antibody
    • Homeobox protein Meis1 antibody
    • Leukemogenic homolog protein antibody
    • MEIS 1 antibody
    • Meis homeo box 1 antibody
    • Meis homeobox 1 antibody
    • Meis1 antibody
    • Meis1 mouse homolog antibody
    • Meis1 myeloid ecotropic viral integration site 1 homolog antibody
    • Meis1 myeloid ecotropic viral integration site 1 homolog mouse antibody
    • MEIS1 protein antibody
    • MEIS1_HUMAN antibody
    • MGC43380 antibody
    • Myeloid ecotropic viral integration site 1 homolog antibody
    • WUGSC:H NH0444B04.1 antibody
    see all

Anti-MEIS1 antibody - ChIP Grade images

  • Anti-MEIS1 antibody - ChIP Grade (ab19867) at 1 µg/ml (blocked with 5% milk) + KG-1 (Human myeloid cell line) Nuclear Lysate at 15 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Performed under reducing conditions.

    Predicted band size : 43, 50 kDa
    Observed band size : 40,48 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 58 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 12 minutes
  • Anti-MEIS1 antibody - ChIP Grade (ab19867) at 1 µg/ml + HEK293 Wcl Transiently Overexpressing MEIS1 at 5 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) (ab65484) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 43, 50 kDa
    Observed band size : 45 kDa (why is the actual band size different from the predicted?)
  • IHC image of MEIS1 staining in Rat normal brain formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab19867, 0.5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-MEIS1 antibody - ChIP Grade (ab19867)

This product has been referenced in:
  • Huang Y  et al. Identification and characterization of Hoxa9 binding sites in hematopoietic cells. Blood 119:388-98 (2012). Read more (PubMed: 22072553) »
  • Sérandour AA  et al. Dynamic hydroxymethylation of deoxyribonucleic acid marks differentiation-associated enhancers. Nucleic Acids Res 40:8255-65 (2012). CHIPseq ; Mouse . Read more (PubMed: 22730288) »

See all 10 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (H9 cell line upon differentiation)
Specification H9 cell line upon differentiation
Blocking step Serum as blocking agent for 10 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative Paraformaldehyde
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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Kidney)
Loading amount 30 µg
Specification Kidney
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
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Verified customer

Submitted Dec 30 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"