Overview

  • Product nameAnti-MEIS1 antibody [EPR5781]
    See all MEIS1 primary antibodies
  • Description
    Rabbit monoclonal [EPR5781] to MEIS1
  • SpecificityThe immunogen of ab124686 is, according to BLAST results, conserved (57%) with human MEIS2. According to this, the antibody might cross-react with MEIS2 but this has, to our knowledge, not been experimentally tested so far.
  • Tested applicationsSuitable for: WB, IP, Flow Cytmore details
    Unsuitable for: ICC or IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide, corresponding to a sequence within Human MEIS1.

  • Positive control
    • U-87 MG, K562, and HeLa cell lysates; permeabilized K562 cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Properties

Applications

Our Abpromise guarantee covers the use of ab124686 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 47 kDa (predicted molecular weight: 43 kDa).
IP 1/10 - 1/100.
Flow Cyt 1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • Application notesIs unsuitable for ICC or IHC-P.
  • Target

    • FunctionActs as a transcriptional regulator of PAX6. Acts as a transcriptional activator of PF4 in complex with PBX1 or PBX2. Required for hematopoiesis, megakaryocyte lineage development and vascular patterning. May function as a cofactor for HOXA7 and HOXA9 in the induction of myeloid leukemias.
    • Tissue specificityExpressed at low level in normal immunohepatopoietic tissues, including the fetal liver. Expressed in a subset of myeloid leukemia cell lines, with the highest expression seen in those with a megakaryocytic-erythroid phenotype. Also expressed at high levels in the cerebellum.
    • Involvement in diseaseDefects in MEIS1 could be a cause of susceptibility to restless legs syndrome type 7 (RLS7) [MIM:612853]. Restless legs syndrome (RLS) is a neurologic sleep/wake disorder characterized by uncomfortable and unpleasant sensations in the legs that appear at rest, usually at night, inducing an irresistible desire to move the legs. The disorder results in nocturnal insomnia and chronic sleep deprivation.
    • Sequence similaritiesBelongs to the TALE/MEIS homeobox family.
      Contains 1 homeobox DNA-binding domain.
    • Cellular localizationNucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • Homeo box protein Meis1 antibody
      • Homeobox protein Meis1 antibody
      • Leukemogenic homolog protein antibody
      • MEIS 1 antibody
      • Meis homeo box 1 antibody
      • Meis homeobox 1 antibody
      • Meis1 antibody
      • Meis1 mouse homolog antibody
      • Meis1 myeloid ecotropic viral integration site 1 homolog antibody
      • Meis1 myeloid ecotropic viral integration site 1 homolog mouse antibody
      • MEIS1 protein antibody
      • MEIS1_HUMAN antibody
      • MGC43380 antibody
      • Myeloid ecotropic viral integration site 1 homolog antibody
      • WUGSC:H NH0444B04.1 antibody
      see all

    Anti-MEIS1 antibody [EPR5781] images

    • All lanes : Anti-MEIS1 antibody [EPR5781] (ab124686) at 1/10000 dilution

      Lane 1 : U-87 MG cell lysate
      Lane 2 : K562 cell lysate
      Lane 3 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 43 kDa
      Observed band size : 47 kDa (why is the actual band size different from the predicted?)
    • ab124686 at 1/10 dilution staining MEIS1 in permeabilized K562 by Flow cytometry (red). Rabbit IgG negative control (green).
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References for Anti-MEIS1 antibody [EPR5781] (ab124686)

    ab124686 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab124686.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"