Overview

  • Product nameAnti-MEK1 + MEK2 antibody [3D9]See all MEK1 + MEK2 primary antibodies ...
  • Description
    Mouse monoclonal [3D9] to MEK1 + MEK2
  • SpecificityRecognizes human MEK1 and MEK2.
  • Tested applicationsWB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Native MEK and recombinant MEK1

  • Positive control
    • Brain. Jurkat cells.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • PurityProtein G purified
  • Clonality Monoclonal
  • Clone number3D9
  • IsotypeIgG1
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab69502 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
IHC-P
  • Application notesELISA: Use at an assay dependent dilution.
    IHC-P: Use at a concentration of 1 µg/ml. Perform heat mediated antigen retrieval by boiling tissue sections in 10 mM citrate, pH 6.0 for 10 min followed by cooling at RT for 20 minbefore commencing with IHC staining protocol.
    WB: Use at a concentration of 0.5 µg/ml. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceMitogen activated protein kinase kinase 1 + 2 (MAPKK1 + MAPKK2) are members of a protein kinase family that activate the ERK 1 + 2/MAPK enzymes by phosphorylating both residues within the threonine – glutamic acid – tyrosine (TEY) motif in the activation loop. MEK 1 + 2 are also activated by dual-phosphorylation, which occurs on serines 218 and 222, in the activation loop of the MEKs.
    • Cellular localizationCytoplasmic
    • Database links
    • Alternative names
      • AA589381 antibody
      • Dual specificity mitogen activated protein kinase kinase 2 antibody
      • Dual specificity mitogen-activated protein kinase kinase 1 antibody
      • Dual specificity mitogen-activated protein kinase kinase 2 antibody
      • EC 2.7.12.2 antibody
      • ERK activator kinase 1 antibody
      • ERK activator kinase 2 antibody
      • FLJ26075 antibody
      • MAP kinase kinase 1 antibody
      • MAP kinase kinase 2 antibody
      • MAP2K 2 antibody
      • MAP2K1 antibody
      • MAP2K2 antibody
      • MAPK / ERK kinase 2 antibody
      • MAPK/ERK kinase 1 antibody
      • MAPK/ERK kinase 2 antibody
      • MAPKK 1 antibody
      • MAPKK 2 antibody
      • MAPKK1 antibody
      • MAPKK2 antibody
      • MEKK1 antibody
      • Mitogen Activated Protein Kinase 1 and 2 antibody
      • Mitogen activated protein kinase kinase 1 antibody
      • Mitogen activated protein kinase kinase 2 antibody
      • Mitogen activated protein kinase kinase 2 p45 antibody
      • Mitogen-activated protein kinase kinase 2, p45 antibody
      • MK2 antibody
      • MKK 1 antibody
      • MKK 2 antibody
      • MKK1 antibody
      • MKK2 antibody
      • PRKMK 1 antibody
      • PRKMK 2 antibody
      • Prkmk1 antibody
      • Prkmk2 antibody
      • protein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1) antibody
      • Protein kinase, mitogen-activated, kinase 1 antibody
      • Protein kinase, mitogen-activated, kinase 2 antibody
      see all

    Anti-MEK1 + MEK2 antibody [3D9] images

    • All lanes : Anti-MEK1 + MEK2 antibody [3D9] (ab69502) at 0.5 µg/ml

      Lane 1 : Jurkat whole cell lysates at 20 µg
      Lane 2 : Jurkat whole cell lysates at 40 µg
      Lane 3 : recombinant MEK2 inactive at 0.02 µg
      Lane 4 : recombinant MEK1 inactive at 0.02 µg
      Lane 5 : recombinant MEK1 active at 0.02 µg
      Lane 6 : recombinant MEK2 active at 0.02 µg
      Lane 7 : MW ladder

      Secondary
      HRP-conjugated goat anti-mouse secondary antibody
      developed using the ECL technique

      Predicted band size : 43 kDa
      Observed band size : 43 or 71 kDa (why is the actual band size different from the predicted?)
    • Formalin-fixed, paraffin-embedded brain tissue stained with 1 µg/mL anti-MEK ab69502.

    References for Anti-MEK1 + MEK2 antibody [3D9] (ab69502)

    ab69502 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab69502.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"