Overview

  • Product nameAnti-MEK1 + MEK2 antibody [3D9]
    See all MEK1 + MEK2 primary antibodies
  • Description
    Mouse monoclonal [3D9] to MEK1 + MEK2
  • SpecificityRecognizes human MEK1 and MEK2.
  • Tested applicationsWB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Native MEK and recombinant MEK1

  • Positive control
    • Brain. Jurkat cells.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • PurityProtein G purified
  • Clonality Monoclonal
  • Clone number3D9
  • IsotypeIgG1
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab69502 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
IHC-P
  • Application notesELISA: Use at an assay dependent dilution.
    IHC-P: Use at a concentration of 1 µg/ml. Perform heat mediated antigen retrieval by boiling tissue sections in 10 mM citrate, pH 6.0 for 10 min followed by cooling at RT for 20 minbefore commencing with IHC staining protocol.
    WB: Use at a concentration of 0.5 µg/ml. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionDual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Binding of extracellular ligands such as growth factors, cytokines and hormones to their cell-surface receptors activates RAS and this initiates RAF1 activation. RAF1 then further activates the dual-specificity protein kinases MAP2K1/MEK1 and MAP2K2/MEK2. Both MAP2K1/MEK1 and MAP2K2/MEK2 function specifically in the MAPK/ERK cascade, and catalyze the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in the extracellular signal-regulated kinases MAPK3/ERK1 and MAPK1/ERK2, leading to their activation and further transduction of the signal within the MAPK/ERK cascade. Depending on the cellular context, this pathway mediates diverse biological functions such as cell growth, adhesion, survival and differentiation, predominantly through the regulation of transcription, metabolism and cytoskeletal rearrangements. One target of the MAPK/ERK cascade is peroxisome proliferator-activated receptor gamma (PPARG), a nuclear receptor that promotes differentiation and apoptosis. MAP2K1/MEK1 has been shown to export PPARG from the nucleus. The MAPK/ERK cascade is also involved in the regulation of endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC), as well as in the fragmentation of the Golgi apparatus during mitosis.
    • Tissue specificityWidely expressed, with extremely low levels in brain.
    • Involvement in diseaseCardiofaciocutaneous syndrome 3
    • Sequence similaritiesBelongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
      Contains 1 protein kinase domain.
    • DomainThe proline-rich region localized between residues 270 and 307 is important for binding to RAF1 and activation of MAP2K1/MEK1.
    • Post-translational
      modifications
      Phosphorylation at Ser-218 and Ser-222 by MAP kinase kinase kinases (RAF or MEKK1) positively regulates kinase activity. Also phosphorylated at Thr-292 by MAPK1/ERK2 and at Ser-298 by PAK. MAPK1/ERK2 phosphorylation of Thr-292 occurs in response to cellular adhesion and leads to inhibition of Ser-298 phosphorylation by PAK.
      Acetylation by Yersinia yopJ prevents phosphorylation and activation, thus blocking the MAPK signaling pathway.
    • Cellular localizationCytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, microtubule organizing center, spindle pole body. Cytoplasm. Nucleus. Localizes at centrosomes during prometaphase, midzone during anaphase and midbody during telophase/cytokinesis.
    • Information by UniProt
    • Database links
    • Alternative names
      • AA589381 antibody
      • Dual specificity mitogen activated protein kinase kinase 2 antibody
      • Dual specificity mitogen-activated protein kinase kinase 1 antibody
      • Dual specificity mitogen-activated protein kinase kinase 2 antibody
      • EC 2.7.12.2 antibody
      • ERK activator kinase 1 antibody
      • ERK activator kinase 2 antibody
      • FLJ26075 antibody
      • MAP kinase kinase 1 antibody
      • MAP kinase kinase 2 antibody
      • MAP2K 2 antibody
      • MAP2K1 antibody
      • MAP2K2 antibody
      • MAPK / ERK kinase 2 antibody
      • MAPK/ERK kinase 1 antibody
      • MAPK/ERK kinase 2 antibody
      • MAPKK 1 antibody
      • MAPKK 2 antibody
      • MAPKK1 antibody
      • MAPKK2 antibody
      • MEK 1 antibody
      • MEKK1 antibody
      • Mitogen Activated Protein Kinase 1 and 2 antibody
      • Mitogen activated protein kinase kinase 1 antibody
      • Mitogen activated protein kinase kinase 2 antibody
      • Mitogen activated protein kinase kinase 2 p45 antibody
      • Mitogen-activated protein kinase kinase 2, p45 antibody
      • MK2 antibody
      • MKK 1 antibody
      • MKK 2 antibody
      • MKK1 antibody
      • MKK2 antibody
      • MP2K1_HUMAN antibody
      • PRKMK 1 antibody
      • PRKMK 2 antibody
      • Prkmk1 antibody
      • Prkmk2 antibody
      • protein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1) antibody
      • Protein kinase, mitogen-activated, kinase 1 antibody
      • Protein kinase, mitogen-activated, kinase 2 antibody
      see all

    Anti-MEK1 + MEK2 antibody [3D9] images

    • All lanes : Anti-MEK1 + MEK2 antibody [3D9] (ab69502) at 0.5 µg/ml

      Lane 1 : Jurkat whole cell lysates at 20 µg
      Lane 2 : Jurkat whole cell lysates at 40 µg
      Lane 3 : recombinant MEK2 inactive at 0.02 µg
      Lane 4 : recombinant MEK1 inactive at 0.02 µg
      Lane 5 : recombinant MEK1 active at 0.02 µg
      Lane 6 : recombinant MEK2 active at 0.02 µg
      Lane 7 : MW ladder

      Secondary
      HRP-conjugated goat anti-mouse secondary antibody
      developed using the ECL technique

      Predicted band size : 43 kDa
      Observed band size : 43 or 71 kDa (why is the actual band size different from the predicted?)
    • Formalin-fixed, paraffin-embedded brain tissue stained with 1 µg/mL anti-MEK ab69502.

    References for Anti-MEK1 + MEK2 antibody [3D9] (ab69502)

    ab69502 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"