Anti-MEK1 + MEK2 antibody (ab70613)

Overview

  • Product nameAnti-MEK1 + MEK2 antibodySee all MEK1 + MEK2 primary antibodies ...
  • Description
    Rabbit polyclonal to MEK1 + MEK2
  • Specificityab70613 recognizes human MEK1 protein without phosphorylation at sites Ser 218/222 and MEK2 without phosphorylation at sites Ser 222/226. It does not cross react to phosphorylated MEK1/2.
  • Tested applicationsWB, IP, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Chicken, Human
  • Immunogen

    Synthetic peptide (Human) surrounding the epitope SMANS without phosphorylation.

  • Positive control
    • Whole cell lysates derived from 3T3 cells.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: Tris buffered saline, pH 7.2 containing antibody stabilizer
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab70613 is purified by site specific epitope affinity purification.
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab70613 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 1 µg/ml. Detects a band of approximately 44 kDa (predicted molecular weight: 44 kDa).
IP Use a concentration of 2 - 5 µg/ml.
ELISA Use a concentration of 0.01 - 0.1 µg/ml.

Target

  • RelevanceMitogen activated protein kinase kinase 1 + 2 (MAPKK1 + MAPKK2) are members of a protein kinase family that activate the ERK 1 + 2/MAPK enzymes by phosphorylating both residues within the threonine – glutamic acid – tyrosine (TEY) motif in the activation loop. MEK 1 + 2 are also activated by dual-phosphorylation, which occurs on serines 218 and 222, in the activation loop of the MEKs.
  • Cellular localizationCytoplasmic
  • Database links
  • Alternative names
    • AA589381 antibody
    • Dual specificity mitogen-activated protein kinase kinase 1 antibody
    • Dual specificity mitogen-activated protein kinase kinase 2 antibody
    • EC 2.7.12.2 antibody
    • ERK activator kinase 1 antibody
    • ERK activator kinase 2 antibody
    • FLJ26075 antibody
    • MAP kinase kinase 1 antibody
    • MAP kinase kinase 2 antibody
    • MAP2K1 antibody
    • MAP2K2 antibody
    • MAPK/ERK kinase 1 antibody
    • MAPK/ERK kinase 2 antibody
    • MAPKK1 antibody
    • MAPKK2 antibody
    • MEKK1 antibody
    • Mitogen activated protein kinase kinase 1 antibody
    • Mitogen activated protein kinase kinase 2 antibody
    • Mitogen-activated protein kinase kinase 2, p45 antibody
    • MK2 antibody
    • MKK 1 antibody
    • MKK 2 antibody
    • MKK1 antibody
    • MKK2 antibody
    • PRKMK 1 antibody
    • PRKMK 2 antibody
    • Prkmk1 antibody
    • Prkmk2 antibody
    • protein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1) antibody
    • Protein kinase, mitogen-activated, kinase 1 antibody
    • Protein kinase, mitogen-activated, kinase 2 antibody
    see all

Anti-MEK1 + MEK2 antibody images

  • Anti-MEK1 + MEK2 antibody (ab70613) at 1/1000 dilution + Whole cell lysates derived from 3T3

    Predicted band size : 44 kDa
    Observed band size : 44 kDa
  • 1 mg peptide was spotted onto NC membrane and blotted using ab70613 at 1/1000 dilution. A:MEK1/2 (pS218/222) B: MEK1/2 (Non-phosphorylated), C: Non-related phosphospecific PP.

References for Anti-MEK1 + MEK2 antibody (ab70613)

ab70613 has not yet been referenced specifically in any publications.

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