RabMAb

Anti-MEK1 + MEK2 (phospho S218 + S222 + S226) antibody [UP42] (ab78132)

Overview

  • Product nameAnti-MEK1 + MEK2 (phospho S218 + S222 + S226) antibody [UP42]See all MEK1 + MEK2 primary antibodies ...
  • Description
    Rabbit monoclonal [UP42] to MEK1 + MEK2 (phospho S218 + S222 + S226)
  • SpecificityRecognizes phosphorylated MEK1(Ser218/222) + MEK2 (Ser222/226)
  • Tested applicationsWB more details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide: DSMANSF conjugated to KLH, corresponding to amino acids 217-223 of Human MEK1 + MEK2. The immunizing sequence is highly conserved.

  • Positive control
    • RIPA lysates from PC-12 cells

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.05% Sodium Azide
    Constituents: 30% Glycerol, 0.15M Sodium chloride, 0.1M Tris glycine, pH 7.4
  • Concentration information loading...
  • PurityProtein A purified
  • Clonality Monoclonal
  • Clone numberUP42
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab78132 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB 1/2000 - 1/10000. Detects a band of approximately 50 kDa (predicted molecular weight: 44 kDa).

Target

  • RelevanceMitogen activated protein kinase kinase 1 + 2 (MAPKK1 + MAPKK2) are members of a protein kinase family that activate the ERK 1 + 2/MAPK enzymes by phosphorylating both residues within the threonine – glutamic acid – tyrosine (TEY) motif in the activation loop. MEK 1 + 2 are also activated by dual-phosphorylation, which occurs on serines 218 and 222, in the activation loop of the MEKs.
  • Cellular localizationCytoplasmic
  • Database links
  • Alternative names
    • AA589381 antibody
    • Dual specificity mitogen activated protein kinase kinase 1 antibody
    • Dual specificity mitogen activated protein kinase kinase 2 antibody
    • Dual specificity mitogen-activated protein kinase kinase 1 antibody
    • Dual specificity mitogen-activated protein kinase kinase 2 antibody
    • EC 2.7.12.2 antibody
    • ERK activator kinase 1 antibody
    • ERK activator kinase 2 antibody
    • FLJ26075 antibody
    • MAP kinase kinase 1 antibody
    • MAP kinase kinase 2 antibody
    • MAP2K 2 antibody
    • MAP2K1 antibody
    • MAP2K2 antibody
    • MAPK / ERK kinase 2 antibody
    • MAPK/ERK kinase 1 antibody
    • MAPK/ERK kinase 2 antibody
    • MAPKK 1 antibody
    • MAPKK 2 antibody
    • MAPKK1 antibody
    • MAPKK2 antibody
    • MEK 1 antibody
    • MEK 2 antibody
    • MEKK1 antibody
    • Mitogen Activated Protein Kinase 1 and 2 antibody
    • Mitogen activated protein kinase kinase 1 antibody
    • Mitogen activated protein kinase kinase 2 antibody
    • Mitogen activated protein kinase kinase 2 p45 antibody
    • Mitogen-activated protein kinase kinase 2, p45 antibody
    • MK2 antibody
    • MKK 1 antibody
    • MKK 2 antibody
    • MKK1 antibody
    • MKK2 antibody
    • PRKMK 1 antibody
    • PRKMK 2 antibody
    • PRKMK1 antibody
    • PRKMK2 antibody
    • Protein kinase mitogen activated kinase 1 antibody
    • protein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1) antibody
    • Protein kinase, mitogen-activated, kinase 1 antibody
    • Protein kinase, mitogen-activated, kinase 2 antibody
    see all

Anti-MEK1 + MEK2 (phospho S218 + S222 + S226) antibody [UP42] images

  • All lanes : Anti-MEK1 + MEK2 (phospho S218 + S222 + S226) antibody [UP42] (ab78132) at 1/5000 dilution

    Lane 1 : PC-12 cell lysate
    Lane 2 : PC-12 cell lysate treated with 5 Units of B-Raf for 30 minutes at 30°C.

    Secondary
    HRP-goat anti-rabbit secondary at 1/5000 dilution

    Predicted band size : 44 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)

References for Anti-MEK1 + MEK2 (phospho S218 + S222 + S226) antibody [UP42] (ab78132)

ab78132 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab78132.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"