Anti-MEK1 + MEK2 (phospho S222) antibody (ab4750)

Overview

  • Product nameAnti-MEK1 + MEK2 (phospho S222) antibody
    See all MEK1 + MEK2 primary antibodies
  • Description
    Rabbit polyclonal to MEK1 + MEK2 (phospho S222)
  • Tested applicationsWBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Chicken, Xenopus laevis, Chimpanzee
  • Immunogen

    Synthetic peptide (Human) derived from a region of human MEK 1 + 2 that contains serine 222. The sequence is conserved in many species including mouse, rat, chimp, chicken, frog, and others.

  • Positive control
    • NIH3T3 cells +/- PDGF, A431 +/- EGF, PC12 +/- NGF.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.05% Sodium Azide
    Constituents: PBS, 1.0mg/ml BSA (IgG, protease free). pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesPurified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated MEK 1 + 2. The final product is generated by affinity chromatography using a MEK 1 + 2 derived peptide that is phosphorylated at serine 222.
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab4750 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWB: Use at a concentration of 0.1 - 1.0 µg/ml.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceMitogen activated protein kinase kinase 1 + 2 (MAPKK1 + MAPKK2) are members of a protein kinase family that activate the ERK 1 + 2/MAPK enzymes by phosphorylating both residues within the threonine – glutamic acid – tyrosine (TEY) motif in the activation loop. MEK 1 + 2 are also activated by dual-phosphorylation, which occurs on serines 218 and 222, in the activation loop of the MEKs.
    • Cellular localizationCytoplasmic
    • Database links
    • Alternative names
      • AA589381 antibody
      • Dual specificity mitogen-activated protein kinase kinase 1 antibody
      • Dual specificity mitogen-activated protein kinase kinase 2 antibody
      • EC 2.7.12.2 antibody
      • ERK activator kinase 1 antibody
      • ERK activator kinase 2 antibody
      • FLJ26075 antibody
      • MAP kinase kinase 1 antibody
      • MAP kinase kinase 2 antibody
      • MAP2K1 antibody
      • MAP2K2 antibody
      • MAPK/ERK kinase 1 antibody
      • MAPK/ERK kinase 2 antibody
      • MAPKK1 antibody
      • MAPKK2 antibody
      • MEKK1 antibody
      • Mitogen Activated Protein Kinase 1 and 2 antibody
      • Mitogen activated protein kinase kinase 1 antibody
      • Mitogen activated protein kinase kinase 2 antibody
      • Mitogen-activated protein kinase kinase 2, p45 antibody
      • MK2 antibody
      • MKK 1 antibody
      • MKK 2 antibody
      • MKK1 antibody
      • MKK2 antibody
      • PRKMK 1 antibody
      • PRKMK 2 antibody
      • Prkmk1 antibody
      • Prkmk2 antibody
      • protein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1) antibody
      • Protein kinase, mitogen-activated, kinase 1 antibody
      • Protein kinase, mitogen-activated, kinase 2 antibody
      see all

    Anti-MEK1 + MEK2 (phospho S222) antibody images



    • Predicted band size : 43.3 kDa


      Extracts prepared from NIH3T3 cells left untreated (lane 1) or treated (lanes 2-5) with PDGF were resolved by SDS-PAGE on a 10% Tris-glycine gel and transferred to nitrocellulose. Membranes were blocked with a 5% BSA TBST buffer overnight at 4oC, then were incubated with 0.50 µg/mL MEK 1 & 2 [pS222] antibody, following prior incubation with: no peptide (1, 2) the nonphosphopeptide corresponding to the immunogen (3), a dual phosphopeptide corresponding to MEK 1 & 2 [pSpS218/222] (4), the phosphopeptide immunogen (5). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG alkaline phosphatase and signals were detected using the Tropix WesternStar method. The data show that the peptides corresponding to MEK 1 & 2 [pS222] and MEK 1 & 2 [pSpS218/222] block the antibody signal, but the corresponding nonphospho-peptide does not, thereby demonstrating the specificity of the antibody. The data also show the induction of the MEK 1 & 2 [pS222] phospho signal upon stimulation with PDGF (compare lanes 1 and 2).

    References for Anti-MEK1 + MEK2 (phospho S222) antibody (ab4750)

    ab4750 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for your email. On the online datasheet for ab4750 there is a nice Western showing results with the peptide competition experiment. As you can see, incubation with the the nonphosphopeptide corresponding to the immunogen does not block the an...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"