Anti-MEK1 + MEK2 (phospho S222) antibody (ab4750)

Overview

  • Product nameAnti-MEK1 + MEK2 (phospho S222) antibody
    See all MEK1 + MEK2 primary antibodies
  • Description
    Rabbit polyclonal to MEK1 + MEK2 (phospho S222)
  • Tested applicationsWBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Chicken, Xenopus laevis, Chimpanzee
  • Immunogen

    Synthetic peptide (Human) derived from a region of human MEK 1 + 2 that contains serine 222. The sequence is conserved in many species including mouse, rat, chimp, chicken, frog, and others.

  • Positive control
    • NIH3T3 cells +/- PDGF, A431 +/- EGF, PC12 +/- NGF.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.05% Sodium Azide
    Constituents: PBS, 1.0mg/ml BSA (IgG, protease free). pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesPurified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated MEK 1 + 2. The final product is generated by affinity chromatography using a MEK 1 + 2 derived peptide that is phosphorylated at serine 222.
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab4750 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWB: Use at a concentration of 0.1 - 1.0 µg/ml.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionDual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Binding of extracellular ligands such as growth factors, cytokines and hormones to their cell-surface receptors activates RAS and this initiates RAF1 activation. RAF1 then further activates the dual-specificity protein kinases MAP2K1/MEK1 and MAP2K2/MEK2. Both MAP2K1/MEK1 and MAP2K2/MEK2 function specifically in the MAPK/ERK cascade, and catalyze the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in the extracellular signal-regulated kinases MAPK3/ERK1 and MAPK1/ERK2, leading to their activation and further transduction of the signal within the MAPK/ERK cascade. Depending on the cellular context, this pathway mediates diverse biological functions such as cell growth, adhesion, survival and differentiation, predominantly through the regulation of transcription, metabolism and cytoskeletal rearrangements. One target of the MAPK/ERK cascade is peroxisome proliferator-activated receptor gamma (PPARG), a nuclear receptor that promotes differentiation and apoptosis. MAP2K1/MEK1 has been shown to export PPARG from the nucleus. The MAPK/ERK cascade is also involved in the regulation of endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC), as well as in the fragmentation of the Golgi apparatus during mitosis.
    • Tissue specificityWidely expressed, with extremely low levels in brain.
    • Involvement in diseaseCardiofaciocutaneous syndrome 3
    • Sequence similaritiesBelongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
      Contains 1 protein kinase domain.
    • DomainThe proline-rich region localized between residues 270 and 307 is important for binding to RAF1 and activation of MAP2K1/MEK1.
    • Post-translational
      modifications
      Phosphorylation at Ser-218 and Ser-222 by MAP kinase kinase kinases (RAF or MEKK1) positively regulates kinase activity. Also phosphorylated at Thr-292 by MAPK1/ERK2 and at Ser-298 by PAK. MAPK1/ERK2 phosphorylation of Thr-292 occurs in response to cellular adhesion and leads to inhibition of Ser-298 phosphorylation by PAK.
      Acetylation by Yersinia yopJ prevents phosphorylation and activation, thus blocking the MAPK signaling pathway.
    • Cellular localizationCytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, microtubule organizing center, spindle pole body. Cytoplasm. Nucleus. Localizes at centrosomes during prometaphase, midzone during anaphase and midbody during telophase/cytokinesis.
    • Information by UniProt
    • Database links
    • Alternative names
      • AA589381 antibody
      • Dual specificity mitogen-activated protein kinase kinase 1 antibody
      • Dual specificity mitogen-activated protein kinase kinase 2 antibody
      • EC 2.7.12.2 antibody
      • ERK activator kinase 1 antibody
      • ERK activator kinase 2 antibody
      • FLJ26075 antibody
      • MAP kinase kinase 1 antibody
      • MAP kinase kinase 2 antibody
      • MAP2K1 antibody
      • MAP2K2 antibody
      • MAPK/ERK kinase 1 antibody
      • MAPK/ERK kinase 2 antibody
      • MAPKK 1 antibody
      • MAPKK1 antibody
      • MAPKK2 antibody
      • MEK 1 antibody
      • MEKK1 antibody
      • Mitogen Activated Protein Kinase 1 and 2 antibody
      • Mitogen activated protein kinase kinase 1 antibody
      • Mitogen activated protein kinase kinase 2 antibody
      • Mitogen-activated protein kinase kinase 2, p45 antibody
      • MK2 antibody
      • MKK 1 antibody
      • MKK 2 antibody
      • MKK1 antibody
      • MKK2 antibody
      • MP2K1_HUMAN antibody
      • PRKMK 1 antibody
      • PRKMK 2 antibody
      • Prkmk1 antibody
      • Prkmk2 antibody
      • protein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1) antibody
      • Protein kinase, mitogen-activated, kinase 1 antibody
      • Protein kinase, mitogen-activated, kinase 2 antibody
      see all

    Anti-MEK1 + MEK2 (phospho S222) antibody images



    • Predicted band size : 43.3 kDa


      Extracts prepared from NIH3T3 cells left untreated (lane 1) or treated (lanes 2-5) with PDGF were resolved by SDS-PAGE on a 10% Tris-glycine gel and transferred to nitrocellulose. Membranes were blocked with a 5% BSA TBST buffer overnight at 4oC, then were incubated with 0.50 µg/mL MEK 1 & 2 [pS222] antibody, following prior incubation with: no peptide (1, 2) the nonphosphopeptide corresponding to the immunogen (3), a dual phosphopeptide corresponding to MEK 1 & 2 [pSpS218/222] (4), the phosphopeptide immunogen (5). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG alkaline phosphatase and signals were detected using the Tropix WesternStar method. The data show that the peptides corresponding to MEK 1 & 2 [pS222] and MEK 1 & 2 [pSpS218/222] block the antibody signal, but the corresponding nonphospho-peptide does not, thereby demonstrating the specificity of the antibody. The data also show the induction of the MEK 1 & 2 [pS222] phospho signal upon stimulation with PDGF (compare lanes 1 and 2).

    References for Anti-MEK1 + MEK2 (phospho S222) antibody (ab4750)

    ab4750 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for your email. On the online datasheet for ab4750 there is a nice Western showing results with the peptide competition experiment. As you can see, incubation with the the nonphosphopeptide corresponding to the immunogen does not block the an...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"