Overview

  • Product nameAnti-MEK1 antibody [7F1]
    See all MEK1 primary antibodies
  • Description
    Mouse monoclonal [7F1] to MEK1
  • SpecificityDoes not bind MEK2.
  • Tested applicationsSuitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length Human protein

  • Positive control
    • Human recombinant MEK1 and MEK2.

Properties

Applications

Our Abpromise guarantee covers the use of ab82544 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 43 kDa.
IP Use a concentration of 4 µg/ml.

Target

  • FunctionCatalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates ERK1 and ERK2 MAP kinases.
  • Tissue specificityWidely expressed, with extremely low levels in brain.
  • Involvement in diseaseDefects in MAP2K1 are a cause of cardiofaciocutaneous syndrome (CFC syndrome) [MIM:115150]; also known as cardio-facio-cutaneous syndrome. CFC syndrome is characterized by a distinctive facial appearance, heart defects and mental retardation. Heart defects include pulmonic stenosis, atrial septal defects and hypertrophic cardiomyopathy. Some affected individuals present with ectodermal abnormalities such as sparse, friable hair, hyperkeratotic skin lesions and a generalized ichthyosis-like condition. Typical facial features are similar to Noonan syndrome. They include high forehead with bitemporal constriction, hypoplastic supraorbital ridges, downslanting palpebral fissures, a depressed nasal bridge, and posteriorly angulated ears with prominent helices. The inheritance of CFC syndrome is autosomal dominant.
  • Sequence similaritiesBelongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Phosphorylation on Ser/Thr by MAP kinase kinase kinases (RAF or MEKK1) regulates positively the kinase activity.
    Acetylation by Yersinia yopJ prevents phosphorylation and activation, thus blocking the MAPK signaling pathway.
  • Information by UniProt
  • Database links
  • Alternative names
    • Dual specificity mitogen activated protein kinase kinase 1 antibody
    • Dual specificity mitogen-activated protein kinase kinase 1 antibody
    • ERK activator kinase 1 antibody
    • MAP kinase kinase 1 antibody
    • MAP kinase/Erk kinase 1 antibody
    • MAP2K1 antibody
    • MAPK/ERK kinase 1 antibody
    • MAPKK 1 antibody
    • MAPKK1 antibody
    • MEK 1 antibody
    • Mek1 antibody
    • MEKK1 antibody
    • Mitogen activated protein kinase kinase 1 antibody
    • MKK 1 antibody
    • MKK1 antibody
    • MP2K1_HUMAN antibody
    • PRKMK1 antibody
    • Protein kinase mitogen activated kinase 1 (MAP kinase kinase 1) antibody
    • Protein kinase mitogen activated, kinase 1 antibody
    see all

Anti-MEK1 antibody [7F1] images

  • All lanes : Anti-MEK1 antibody [7F1] (ab82544) at 1/1000 dilution

    Lane 1 : Human MEK1 recombinant protein
    Lane 2 : Human MEK2 recombinant protein

    Lysates/proteins at 0.02 µg per lane.


    Predicted band size : 43 kDa
    Observed band size : 47 kDa (why is the actual band size different from the predicted?)

References for Anti-MEK1 antibody [7F1] (ab82544)

ab82544 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab82544.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"