Synthetic peptide corresponding to Human MEK1 conjugated to Keyhole Limpet Haemocyanin (KLH). Synthetic phosphopeptide corresponding to amino acid residues surrounding S218/222 of human MEK1 conjugated to KLH
Ramos cell line lysate (WB)
Human hepatocarcinoma tissue (IHC)
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Purification notesab60002 is first purified by protein G affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2 step procedure with the control and phosphorylated peptides. The phospho specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/50 - 1/100.
1/100 - 1/500. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).
FunctionCatalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates ERK1 and ERK2 MAP kinases.
Tissue specificityWidely expressed, with extremely low levels in brain.
Involvement in diseaseDefects in MAP2K1 are a cause of cardiofaciocutaneous syndrome (CFC syndrome) [MIM:115150]; also known as cardio-facio-cutaneous syndrome. CFC syndrome is characterized by a distinctive facial appearance, heart defects and mental retardation. Heart defects include pulmonic stenosis, atrial septal defects and hypertrophic cardiomyopathy. Some affected individuals present with ectodermal abnormalities such as sparse, friable hair, hyperkeratotic skin lesions and a generalized ichthyosis-like condition. Typical facial features are similar to Noonan syndrome. They include high forehead with bitemporal constriction, hypoplastic supraorbital ridges, downslanting palpebral fissures, a depressed nasal bridge, and posteriorly angulated ears with prominent helices. The inheritance of CFC syndrome is autosomal dominant.
Sequence similaritiesBelongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily. Contains 1 protein kinase domain.
Post-translational modificationsPhosphorylation on Ser/Thr by MAP kinase kinase kinases (RAF or MEKK1) regulates positively the kinase activity. Acetylation by Yersinia yopJ prevents phosphorylation and activation, thus blocking the MAPK signaling pathway.
ab60002 at 1/50 dilution staining MEK1 in Human hepatocarcinoma by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue.
The primary antibody was peroxidase conjugated to the secondary antibody, followed by AEC staining
Western blot - MEK1 (phospho S217 + S221) antibody (ab60002)
Anti-MEK1 (phospho S218 + S222) antibody (ab60002) at 1/60 dilution + Ramos cell line lysate at 35 µg
Predicted band size : 43 kDa Observed band size : 43 kDa
References for Anti-MEK1 (phospho S218 + S222) antibody (ab60002)
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