The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 39 kDa.
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Use a concentration of 1 µg/ml.
FunctionDual specificity kinase. Is activated by cytokines and environmental stress in vivo. Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in the MAP kinase p38.
Tissue specificityAbundant expression is seen in the skeletal muscle. It is also widely expressed in other tissues.
Involvement in diseaseNote=Defects in MAP2K3 may be involved in colon cancer.
Sequence similaritiesBelongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily. Contains 1 protein kinase domain.
Post-translational modificationsAutophosphorylated. Phosphorylation on Ser-218 and Thr-222 by MAP kinase kinase kinases regulates positively the kinase activity. Yersinia yopJ may acetylate Ser/Thr residues, preventing phosphorylation and activation, thus blocking the MAPK signaling pathway.
dual specificity mitogen activated protein kinase kinase 3 antibody
Dual specificity mitogen-activated protein kinase kinase 3 antibody
MAP kinase kinase 3 antibody
MAPK ERK kinase 3 antibody
MAPK/ERK kinase 3 antibody
MAPKK 3 antibody
MEK 3 antibody
Mitogen activated protein kinase kinase 3 antibody
MKK 3 antibody
PRKMK 3 antibody
protein kinase, mitogen-activated, kinase 3 antibody
SAPK kinase 2 antibody
SAPKK 2 antibody
Stress activated protein kinase kinase 2 antibody
Anti-MEK3 antibody images
Western blot - MEK3 antibody (ab109557)
All lanes : Anti-MEK3 antibody (ab109557) at 1 µg/ml
Lane 1 : Rat spleen tissue lysate Lane 2 : Rat thymus tissue lysate Lane 3 : Rat skeletal muscle tissue lysate Lane 4 : Rat kidney tissue lysate Lane 5 : MCF-7 whole cell lysate Lane 6 : HeLa whole cell lysate Lane 7 : Raji whole cell lysate Lane 8 : CEM whole cell lysate Lane 9 : Colo320 whole cell lysate
Predicted band size : 39 kDa
References for Anti-MEK3 antibody (ab109557)
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