Overview

  • Product nameAnti-MEK3 antibody
    See all MEK3 primary antibodies
  • Description
    Rabbit polyclonal to MEK3
  • SpecificityNo cross reactivity with other proteins.
  • Tested applicationsSuitable for: WB, IHC-P, ICCmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse, Chicken, Cow, Dog
  • Immunogen

    Synthetic peptide:

    MEHPFFTLHKTKKTD

    , corresponding to C terminal amino acids 320-334 of Human MEK3.

  • Positive control
    • Rat spleen tissue lysate, Rat thymus tissue lysate, Rat skeletal muscle tissue lysate, Rat kidney tissue lysate, MCF-7 whole cell lysate, HeLa whole cell lysate, Raji whole cell lysate, CEM whole cell lysate, Colo320 whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab109557 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 39 kDa.
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC Use a concentration of 1 µg/ml.

Target

  • FunctionDual specificity kinase. Is activated by cytokines and environmental stress in vivo. Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in the MAP kinase p38.
  • Tissue specificityAbundant expression is seen in the skeletal muscle. It is also widely expressed in other tissues.
  • Involvement in diseaseNote=Defects in MAP2K3 may be involved in colon cancer.
  • Sequence similaritiesBelongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Autophosphorylated.
    Phosphorylation on Ser-218 and Thr-222 by MAP kinase kinase kinases regulates positively the kinase activity.
    Yersinia yopJ may acetylate Ser/Thr residues, preventing phosphorylation and activation, thus blocking the MAPK signaling pathway.
  • Information by UniProt
  • Database links
  • Alternative names
    • AW212142 antibody
    • dual specificity mitogen activated protein kinase kinase 3 antibody
    • Dual specificity mitogen-activated protein kinase kinase 3 antibody
    • MAP kinase kinase 3 antibody
    • map2k3 antibody
    • MAPK ERK kinase 3 antibody
    • MAPK/ERK kinase 3 antibody
    • MAPKK 3 antibody
    • MAPKK3 antibody
    • MEK 3 antibody
    • MEK3 antibody
    • Mitogen activated protein kinase kinase 3 antibody
    • MKK 3 antibody
    • MKK3 antibody
    • mMKK3b antibody
    • MP2K3_HUMAN antibody
    • PRKMK 3 antibody
    • PRKMK3 antibody
    • protein kinase, mitogen-activated, kinase 3 antibody
    • SAPK kinase 2 antibody
    • SAPKK 2 antibody
    • SAPKK2 antibody
    • Stress activated protein kinase kinase 2 antibody
    see all

Anti-MEK3 antibody images

  • All lanes : Anti-MEK3 antibody (ab109557) at 1 µg/ml

    Lane 1 : Rat spleen tissue lysate
    Lane 2 : Rat thymus tissue lysate
    Lane 3 : Rat skeletal muscle tissue lysate
    Lane 4 : Rat kidney tissue lysate
    Lane 5 : MCF-7 whole cell lysate
    Lane 6 : HeLa whole cell lysate
    Lane 7 : Raji whole cell lysate
    Lane 8 : CEM whole cell lysate
    Lane 9 : Colo320 whole cell lysate


    Predicted band size : 39 kDa

References for Anti-MEK3 antibody (ab109557)

ab109557 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"