Overview

  • Product name
  • Description
    Rabbit polyclonal to MEK3
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow
  • Immunogen

    Recombinant fragment corresponding to a region within amino acids 1-347 of Human MEK3 (Uniprot ID: P46734).

  • Positive control
    • 293T, A431, H1299, HeLa, HepG2, Raji and mouse liver whole cell lysates; HeLa cells; SW480 xenograft tissue.
  • General notes
    Keep as concentrated solution.

Properties

Applications

Our Abpromise guarantee covers the use of ab154913 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 39 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)
ICC/IF 1/100 - 1/1000.

Target

  • Function
    Dual specificity kinase. Is activated by cytokines and environmental stress in vivo. Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in the MAP kinase p38.
  • Tissue specificity
    Abundant expression is seen in the skeletal muscle. It is also widely expressed in other tissues.
  • Involvement in disease
    Note=Defects in MAP2K3 may be involved in colon cancer.
  • Sequence similarities
    Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Autophosphorylated.
    Phosphorylation on Ser-218 and Thr-222 by MAP kinase kinase kinases regulates positively the kinase activity.
    Yersinia yopJ may acetylate Ser/Thr residues, preventing phosphorylation and activation, thus blocking the MAPK signaling pathway.
  • Information by UniProt
  • Database links
  • Alternative names
    • AW212142 antibody
    • dual specificity mitogen activated protein kinase kinase 3 antibody
    • Dual specificity mitogen-activated protein kinase kinase 3 antibody
    • MAP kinase kinase 3 antibody
    • map2k3 antibody
    • MAPK ERK kinase 3 antibody
    • MAPK/ERK kinase 3 antibody
    • MAPKK 3 antibody
    • MAPKK3 antibody
    • MEK 3 antibody
    • MEK3 antibody
    • Mitogen activated protein kinase kinase 3 antibody
    • MKK 3 antibody
    • MKK3 antibody
    • mMKK3b antibody
    • MP2K3_HUMAN antibody
    • PRKMK 3 antibody
    • PRKMK3 antibody
    • protein kinase, mitogen-activated, kinase 3 antibody
    • SAPK kinase 2 antibody
    • SAPKK 2 antibody
    • SAPKK2 antibody
    • Stress activated protein kinase kinase 2 antibody
    see all

Images

  • All lanes : Anti-MEK3 antibody (ab154913) at 1/1000 dilution

    Lane 1 : A431 whole cell lysate
    Lane 2 : Raji whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 39 kDa



    10% SDS PAGE
  • Anti-MEK3 antibody (ab154913) at 1/500 dilution + Mouse liver whole cell lysate at 50 µg

    Predicted band size: 39 kDa



    10% SDS PAGE
  • Immunohistochemical analysis of paraffin-embedded Human SW480 xenograft tissue labeling MEK3 with ab154913 at 1/500 dilution.
  • Immunofluorescence analysis of methanol-fixed HeLa cells labeling MEK3 with ab154913 at 1/200 dilution (green). The image in the lower panel is costained with Hoechst 33342 (blue).

References

ab154913 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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