Anti-MEK3 antibody (ab5428)


  • Product nameAnti-MEK3 antibody
    See all MEK3 primary antibodies
  • Description
    Rabbit polyclonal to MEK3
  • Tested applicationsSuitable for: IHC-P, ELISA, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide:


    conjugated to KLH, corresponding to N terminal amino acids 1-16 of Human MKK3

  • Positive control
    • Jurkat cell lysate.


Associated products


Our Abpromise guarantee covers the use of ab5428 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100.
ELISA 1/1000.
WB 1/100 - 1/500. Detects a band of approximately 35 kDa (predicted molecular weight: 38 kDa).


  • FunctionDual specificity kinase. Is activated by cytokines and environmental stress in vivo. Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in the MAP kinase p38.
  • Tissue specificityAbundant expression is seen in the skeletal muscle. It is also widely expressed in other tissues.
  • Involvement in diseaseNote=Defects in MAP2K3 may be involved in colon cancer.
  • Sequence similaritiesBelongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    Phosphorylation on Ser-218 and Thr-222 by MAP kinase kinase kinases regulates positively the kinase activity.
    Yersinia yopJ may acetylate Ser/Thr residues, preventing phosphorylation and activation, thus blocking the MAPK signaling pathway.
  • Information by UniProt
  • Database links
  • Alternative names
    • AW212142 antibody
    • dual specificity mitogen activated protein kinase kinase 3 antibody
    • Dual specificity mitogen-activated protein kinase kinase 3 antibody
    • MAP kinase kinase 3 antibody
    • map2k3 antibody
    • MAPK ERK kinase 3 antibody
    • MAPK/ERK kinase 3 antibody
    • MAPKK 3 antibody
    • MAPKK3 antibody
    • MEK 3 antibody
    • MEK3 antibody
    • Mitogen activated protein kinase kinase 3 antibody
    • MKK 3 antibody
    • MKK3 antibody
    • mMKK3b antibody
    • MP2K3_HUMAN antibody
    • PRKMK 3 antibody
    • PRKMK3 antibody
    • protein kinase, mitogen-activated, kinase 3 antibody
    • SAPK kinase 2 antibody
    • SAPKK 2 antibody
    • SAPKK2 antibody
    • Stress activated protein kinase kinase 2 antibody
    see all

Anti-MEK3 antibody images

  • Predicted band size : 38 kDa

    ab5428 at a 1/100 dilution staining approximately 35kDa band of MKK 3 in Jurkat cell lysate by Western blot (ECL). ab5428 at a 1/100 dilution staining approximately 35kDa band of MKK 3 in Jurkat cell lysate by Western blot (ECL).  Secondary antibody - anti-rabbit HRP

  • ab5428 staining MEK3 in human breast carcinoma (BC) tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

References for Anti-MEK3 antibody (ab5428)

ab5428 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - other (brain tissue lysate, cytosolic fraction)
Loading amount 10 µg
Specification brain tissue lysate, cytosolic fraction
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 18°C

Dr. Maryna Polyakova

Verified customer

Submitted May 09 2012

Thank you for your reply.
Reviewing the remaining antibodies that are already tested in WB and the species you wish to use, the following do not yet have a western blot image, and I would be pleased to arrange a testing discount for these addition...

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