Validated using a knockout cell line
Recombinant
RabMAb

Anti-MEK3 antibody [EP555Y] (ab40836)

Overview

  • Product name
    Anti-MEK3 antibody [EP555Y]
    See all MEK3 primary antibodies
  • Description
    Rabbit monoclonal [EP555Y] to MEK3
  • Specificity
    This antibody is specific for MEK3.
  • Tested applications
    Suitable for: IP, WB, Flow Cytmore details
    Unsuitable for: ICC or IHC
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to residues in the N-terminus of Human MEK3.

  • Positive control
    • Jurkat, Hela, MCF-7 and K562 cell lysate.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    A trial size is available to purchase for this antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab40836 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP 1/50.
WB 1/5000 - 1/20000. Detects a band of approximately 40 kDa (predicted molecular weight: 39 kDa).
Flow Cyt 1/20.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • Application notes
    Is unsuitable for ICC or IHC.
  • Target

    • Function
      Dual specificity kinase. Is activated by cytokines and environmental stress in vivo. Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in the MAP kinase p38.
    • Tissue specificity
      Abundant expression is seen in the skeletal muscle. It is also widely expressed in other tissues.
    • Involvement in disease
      Note=Defects in MAP2K3 may be involved in colon cancer.
    • Sequence similarities
      Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
      Contains 1 protein kinase domain.
    • Post-translational
      modifications
      Autophosphorylated.
      Phosphorylation on Ser-218 and Thr-222 by MAP kinase kinase kinases regulates positively the kinase activity.
      Yersinia yopJ may acetylate Ser/Thr residues, preventing phosphorylation and activation, thus blocking the MAPK signaling pathway.
    • Information by UniProt
    • Database links
    • Alternative names
      • AW212142 antibody
      • dual specificity mitogen activated protein kinase kinase 3 antibody
      • Dual specificity mitogen-activated protein kinase kinase 3 antibody
      • MAP kinase kinase 3 antibody
      • map2k3 antibody
      • MAPK ERK kinase 3 antibody
      • MAPK/ERK kinase 3 antibody
      • MAPKK 3 antibody
      • MAPKK3 antibody
      • MEK 3 antibody
      • MEK3 antibody
      • Mitogen activated protein kinase kinase 3 antibody
      • MKK 3 antibody
      • MKK3 antibody
      • mMKK3b antibody
      • MP2K3_HUMAN antibody
      • PRKMK 3 antibody
      • PRKMK3 antibody
      • protein kinase, mitogen-activated, kinase 3 antibody
      • SAPK kinase 2 antibody
      • SAPKK 2 antibody
      • SAPKK2 antibody
      • Stress activated protein kinase kinase 2 antibody
      see all

    Images



    • Predicted band size : 39 kDa

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: MEK3knockout HAP1 cell lysate (20 µg)
      Lane 3: HeLa cell lysate (20 µg)
      Lane 4: Jurkat cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab40836 observed at 150 kDa. Red - loading control, ab8245, observed at 37 kDa.
      ab40836 was shown to recognize MEK3 when MEK3 knockout samples were used, along with additional cross-reactive bands. Wild-type and MEK3 knockout samples were subjected to SDS-PAGE. ab40836 and ab8245 (loading control to GAPDH) were both diluted to 1/5000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    • All lanes : Anti-MEK3 antibody [EP555Y] (ab40836) at 1/20000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : Hela cell lysate
      Lane 3 : MCF-7 cell lysate
      Lane 4 : K562 cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 39 kDa
      Observed band size : 40 kDa (why is the actual band size different from the predicted?)

    References

    ab40836 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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