Anti-MEK3 (phospho T222) antibody (ab192659)

Overview

  • Product name
    Anti-MEK3 (phospho T222) antibody
    See all MEK3 primary antibodies
  • Description
    Rabbit polyclonal to MEK3 (phospho T222)
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to Human MEK3 (phospho T222) conjugated to Keyhole Limpet Haemocyanin (KLH). Peptide sequence around phosphorylation site of threonine 222 (A-K-T(p)-M-D). NP_659731.1.
    Database link: P46734

  • Positive control
    • Human brain tissue; Jurkat cell extracts, treated with serum.

Properties

Applications

Our Abpromise guarantee covers the use of ab192659 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 39 kDa.
IHC-P 1/50 - 1/100.

Target

  • Function
    Dual specificity kinase. Is activated by cytokines and environmental stress in vivo. Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in the MAP kinase p38.
  • Tissue specificity
    Abundant expression is seen in the skeletal muscle. It is also widely expressed in other tissues.
  • Involvement in disease
    Note=Defects in MAP2K3 may be involved in colon cancer.
  • Sequence similarities
    Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Autophosphorylated.
    Phosphorylation on Ser-218 and Thr-222 by MAP kinase kinase kinases regulates positively the kinase activity.
    Yersinia yopJ may acetylate Ser/Thr residues, preventing phosphorylation and activation, thus blocking the MAPK signaling pathway.
  • Information by UniProt
  • Database links
  • Alternative names
    • AW212142 antibody
    • dual specificity mitogen activated protein kinase kinase 3 antibody
    • Dual specificity mitogen-activated protein kinase kinase 3 antibody
    • MAP kinase kinase 3 antibody
    • map2k3 antibody
    • MAPK ERK kinase 3 antibody
    • MAPK/ERK kinase 3 antibody
    • MAPKK 3 antibody
    • MAPKK3 antibody
    • MEK 3 antibody
    • MEK3 antibody
    • Mitogen activated protein kinase kinase 3 antibody
    • MKK 3 antibody
    • MKK3 antibody
    • mMKK3b antibody
    • MP2K3_HUMAN antibody
    • PRKMK 3 antibody
    • PRKMK3 antibody
    • protein kinase, mitogen-activated, kinase 3 antibody
    • SAPK kinase 2 antibody
    • SAPKK 2 antibody
    • SAPKK2 antibody
    • Stress activated protein kinase kinase 2 antibody
    see all

Images

  • All lanes : Anti-MEK3 (phospho T222) antibody (ab192659) at 1/500 dilution

    Lane 1 : Jurkat cell extracts, treated with serum
    Lane 2 : Jurkat cell extracts, treated with serum with antigen-specific peptide


    Predicted band size : 39 kDa
  • Immunohistochemical analysis of paraffin embedded Human brain tissue labeling MEK3 with ab192659 at 1/50 dilution. The image on the right has been pre-incubated with blocking peptide.

References

ab192659 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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