Overview

  • Product name
    Anti-MEK4 antibody [EP615Y]
    See all MEK4 primary antibodies
  • Description
    Rabbit monoclonal [EP615Y] to MEK4
  • Tested applications
    Suitable for: WBmore details
    Unsuitable for: ICC
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human MEK4 (C terminal).

  • Positive control
    • HeLa, HEK293, F9, C6, NIH/3T3 cell lysates.
  • General notes

    A trial size is available to purchase for this antibody.

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity
    Protein A purified
  • Purification notes
    ab33912 is a purified IgG.
  • Clonality
    Monoclonal
  • Clone number
    EP615Y
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab33912 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 44 kDa.
  • Application notes
    Is unsuitable for ICC.
  • Target

    • Function
      Dual specificity kinase that activates the JUN kinases MAPK8 (JNK1) and MAPK9 (JNK2) as well as MAPK14 (p38) but not MAPK1 (ERK2) or MAPK3 (ERK1).
    • Tissue specificity
      Abundant expression is seen in the skeletal muscle. It is also widely expressed in other tissues.
    • Sequence similarities
      Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
      Contains 1 protein kinase domain.
    • Post-translational
      modifications
      Activated by phosphorylation on Ser/Thr by MAP kinase kinase kinases.
    • Information by UniProt
    • Database links
    • Alternative names
      • c Jun N terminal kinase kinase 1 antibody
      • C-JUN N-terminal kinase kinase 1 antibody
      • Dual specificity mitogen activated protein kinase kinase 4 antibody
      • Dual specificity mitogen-activated protein kinase kinase 4 antibody
      • JNK Activated Kinase 1 antibody
      • JNK activating kinase 1 antibody
      • JNK-activating kinase 1 antibody
      • JNKK antibody
      • JNKK1 antibody
      • MAP kinase kinase 4 antibody
      • Map2k4 antibody
      • MAPK ERK kinase 4 antibody
      • MAPK/ERK kinase 4 antibody
      • MAPKK 4 antibody
      • MAPKK4 antibody
      • MEK 4 antibody
      • MEK4 antibody
      • Mitogen activated protein kinase kinase 4 antibody
      • MKK 4 antibody
      • MKK4 antibody
      • MP2K4_HUMAN antibody
      • PRKMK4 antibody
      • SAPK ERK kinase 1 antibody
      • SAPK/ERK kinase 1 antibody
      • SAPKK 1 antibody
      • SAPKK1 antibody
      • SEK1 antibody
      • SERK1 antibody
      • SKK1 antibody
      • Stress activated protein kinase kinase 1 antibody
      see all

    Anti-MEK4 antibody [EP615Y] images



    • Predicted band size : 44 kDa

      Lanes 1, 5 and 9: Wild-type HAP1 cell lysate (20 µg)
      Lanes 2, 6 and 10: MEK4 knockout HAP1 cell lysate (20 µg)
      Lanes 3, 7 and 11: HeLa cell lysate (20 µg)
      Lanes 4, 8 and 12: A431 cell lysate (20 µg)
      Lanes 1, 2, 3 and 4: Green signal from target - ab33912 observed at 44 kDa
      Lanes 5, 6, 7 and 8: Red signal from loading control - ab8245 observed at 37 kDa
      Lanes 9, 10, 11 and 12: Merged (red and green) signal

      ab33912 was shown to specifically react with MEK4 when MEK4 knockout samples were used. Wild-type and MEK4 knockout samples were subjected to SDS-PAGE. ab33912 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

    • All lanes : Anti-MEK4 antibody [EP615Y] (ab33912) at 1/5000 dilution (purified)

      Lane 1 : F9 (Mouse embryonal carcinoma epithelial cell) whole cell lysate at 15 µg
      Lane 2 : C6 (Rat glial tumor glial cell) whole cell lysate at 20 µg
      Lane 3 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate at 15 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size : 44 kDa
      Observed band size : 44 kDa

      Blocking and diluting buffer: 5% NFDM/TBST

    • All lanes : Anti-MEK4 antibody [EP615Y] (ab33912) at 1/1000 dilution (purified)

      Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
      Lane 2 : 293 (Human embryonic kidney epithelial cell) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size : 44 kDa
      Observed band size : 44 kDa

      Blocking and diluting buffer: 5% NFDM /TBST

    • Anti-MEK4 antibody [EP615Y] (ab33912) at 1/1000 dilution (unpurified) + HeLa cell lysate

      Predicted band size : 44 kDa
      Observed band size : 44 kDa

    References for Anti-MEK4 antibody [EP615Y] (ab33912)

    This product has been referenced in:
    • Wuestefeld T  et al. A Direct in vivo RNAi screen identifies MKK4 as a key regulator of liver regeneration. Cell 153:389-401 (2013). IF, IHC ; Mouse . Read more (PubMed: 23582328) »

    See 1 Publication for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (LnCap, PC3, HeLa cells)
    Loading amount
    10 µg
    Specification
    LnCap, PC3, HeLa cells
    Gel Running Conditions
    Non-reduced Denaturing
    Blocking step
    Sigma-AldrichWestern Blocker Solution (W0138) as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 4°C
    Username

    Abcam user community

    Verified customer

    Submitted Nov 01 2007

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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