Anti-MEK4 (phospho S80) antibody [EP1075Y] (ab52958)


  • Product nameAnti-MEK4 (phospho S80) antibody [EP1075Y]
    See all MEK4 primary antibodies
  • Description
    Rabbit monoclonal [EP1075Y] to MEK4 (phospho S80)
  • Tested applicationsSuitable for: IHC-P, WB, IPmore details
    Unsuitable for: Flow Cyt or ICC
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    A synthetic peptide corresponding to residues near the N-terminal of human MKK4

  • Positive control
    • 3T3 cell lysates (untreated or treated with PDGF) and human muscle tissue.
  • General notes

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    A trial size is available to purchase for this antibody.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.



Our Abpromise guarantee covers the use of ab52958 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB 1/5000. Detects a band of approximately 44 kDa (predicted molecular weight: 44 kDa).
IP 1/50.
  • Application notesIs unsuitable for Flow Cyt or ICC.
  • Target

    • FunctionDual specificity kinase that activates the JUN kinases MAPK8 (JNK1) and MAPK9 (JNK2) as well as MAPK14 (p38) but not MAPK1 (ERK2) or MAPK3 (ERK1).
    • Tissue specificityAbundant expression is seen in the skeletal muscle. It is also widely expressed in other tissues.
    • Sequence similaritiesBelongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
      Contains 1 protein kinase domain.
    • Post-translational
      Activated by phosphorylation on Ser/Thr by MAP kinase kinase kinases.
    • Information by UniProt
    • Database links
    • Alternative names
      • c Jun N terminal kinase kinase 1 antibody
      • C-JUN N-terminal kinase kinase 1 antibody
      • Dual specificity mitogen activated protein kinase kinase 4 antibody
      • Dual specificity mitogen-activated protein kinase kinase 4 antibody
      • JNK Activated Kinase 1 antibody
      • JNK activating kinase 1 antibody
      • JNK-activating kinase 1 antibody
      • JNKK antibody
      • JNKK1 antibody
      • MAP kinase kinase 4 antibody
      • Map2k4 antibody
      • MAPK ERK kinase 4 antibody
      • MAPK/ERK kinase 4 antibody
      • MAPKK 4 antibody
      • MAPKK4 antibody
      • MEK 4 antibody
      • MEK4 antibody
      • Mitogen activated protein kinase kinase 4 antibody
      • MKK 4 antibody
      • MKK4 antibody
      • MP2K4_HUMAN antibody
      • PRKMK4 antibody
      • SAPK ERK kinase 1 antibody
      • SAPK/ERK kinase 1 antibody
      • SAPKK 1 antibody
      • SAPKK1 antibody
      • SEK1 antibody
      • SERK1 antibody
      • SKK1 antibody
      • Stress activated protein kinase kinase 1 antibody
      see all

    Anti-MEK4 (phospho S80) antibody [EP1075Y] images

    • ab52958 at 1/50 dilution staining MKK4 in human muscles by Immunohistochemistry, Paraffin embedded tissue.
    • All lanes : Anti-MEK4 (phospho S80) antibody [EP1075Y] (ab52958) at 1/5000 dilution

      Lane 1 : 3T3 cell lysate untreated
      Lane 2 : 3T3 cell lysate treated with PDGF

      Lysates/proteins at 10 µg per lane.

      goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 44 kDa
      Observed band size : 44 kDa

    References for Anti-MEK4 (phospho S80) antibody [EP1075Y] (ab52958)

    ab52958 has not yet been referenced specifically in any publications.

    Product Wall

    Application Western blot
    Loading amount 60 µg
    Gel Running Conditions Reduced Denaturing (10)
    Sample Human Cell lysate - whole cell (HepG2)
    Specification HepG2
    Treatment EGF 50ng/ml for 2hrs
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

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    Submitted Nov 17 2014