- Datasheet
- Specific References
- Protocols
Overview
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Product nameAnti-MEK4 (phospho S80) antibody [EP1075Y]
See all MEK4 primary antibodies -
DescriptionRabbit monoclonal [EP1075Y] to MEK4 (phospho S80)
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Host speciesRabbit
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Tested applicationsSuitable for: IHC-P, WB, IPmore details
Unsuitable for: Flow Cyt or ICC -
Species reactivityReacts with: Mouse, Human
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Immunogen
A synthetic peptide corresponding to residues near the N-terminal of human MKK4
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Positive control
- 3T3 cell lysates (untreated or treated with PDGF) and human muscle tissue.
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General notes
A trial size is available to purchase for this antibody.
Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
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Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
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PurityTissue culture supernatant
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ClonalityMonoclonal
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Clone numberEP1075Y
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IsotypeIgG
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Research areas
Applications
Our Abpromise guarantee covers the use of ab52958 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. | |
| WB | 1/5000. Detects a band of approximately 44 kDa (predicted molecular weight: 44 kDa). | |
| IP | 1/50. |
Target
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FunctionDual specificity kinase that activates the JUN kinases MAPK8 (JNK1) and MAPK9 (JNK2) as well as MAPK14 (p38) but not MAPK1 (ERK2) or MAPK3 (ERK1).
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Tissue specificityAbundant expression is seen in the skeletal muscle. It is also widely expressed in other tissues.
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Sequence similaritiesBelongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
Contains 1 protein kinase domain. -
Post-translational
modificationsActivated by phosphorylation on Ser/Thr by MAP kinase kinase kinases. - Information by UniProt
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Database links
- Entrez Gene: 6416 Human
- Entrez Gene: 26398 Mouse
- Omim: 601335 Human
- SwissProt: P45985 Human
- SwissProt: P47809 Mouse
- Unigene: 514681 Human
- Unigene: 412922 Mouse
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Alternative names
- c Jun N terminal kinase kinase 1 antibody
- C-JUN N-terminal kinase kinase 1 antibody
- Dual specificity mitogen activated protein kinase kinase 4 antibody
see all
Images
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEK4 (phospho S80) antibody [EP1075Y] (ab52958)](http://a.abcam.com/ps/products/52/ab52958/Images/ab52958ihc.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEK4 (phospho S80) antibody [EP1075Y] (ab52958)ab52958 at 1/50 dilution staining MKK4 in human muscles by Immunohistochemistry, Paraffin embedded tissue. -
![Western blot - MKK4 (phospho S80) antibody [EP1075Y] (ab52958)](http://a.abcam.com/ps/products/52/ab52958/Images/ab52958wb.gif)
Western blot - MKK4 (phospho S80) antibody [EP1075Y] (ab52958)All lanes : Anti-MEK4 (phospho S80) antibody [EP1075Y] (ab52958) at 1/5000 dilution
Lane 1 : 3T3 cell lysate untreated
Lane 2 : 3T3 cell lysate treated with PDGF
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 44 kDa
Observed band size: 44 kDa
References
ab52958 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"