The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.1 - 1 µg/ml. Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa).
Use a concentration of 2 - 5 µg/ml.
Use a concentration of 0.01 - 0.1 µg/ml.
FunctionActs as a scaffold for the formation of a ternary MAP3K2/MAP3K3-MAP3K5-MAPK7 signaling complex. Activation of this pathway appear to play a critical role in protecting cells from stress-induced apopotosis, neuronal survival and cardiac development and angiogenesis.
Tissue specificityExpressed in many adult tissues. Abundant in heart and skeletal muscle.
Sequence similaritiesBelongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily. Contains 1 OPR domain. Contains 1 protein kinase domain.
DomainBinds MAP3K2/MAP3K3 and MAPK7 via non-overlapping residues of the OPR domain. This domain also mediates interactions with SQSTM1 and PARD6A.
Post-translational modificationsActivated by phosphorylation on Ser/Thr by MAP kinase kinase kinases. Yersinia yopJ may acetylate Ser/Thr residues, preventing phosphorylation and activation, thus blocking the MAPK signaling pathway.
Dual specificity mitogen activated protein kinase kinase 5 antibody
Dual specificity mitogen-activated protein kinase kinase 5 antibody
EC 188.8.131.52 antibody
MAP kinase kinase 5 antibody
MAP kinase kinase MEK5b antibody
MAPK/ERK kinase 5 antibody
MAPKK 5 antibody
MEK 5 antibody
mitogen-activated protein kinase kinase 5 antibody
Protein kinase, mitogen-activated, kinase 5 antibody
Anti-MEK5 antibody images
Western blot - MEK5 antibody (ab70611)
All lanes : Anti-MEK5 antibody (ab70611) at 1/1000 dilution
Lane 1 : Whole cell
lysates derived from mouse
liver Lane 2 : Negative control
Predicted band size : 49 kDa Observed band size : 49 kDa
Dot Blot - MEK5 antibody (ab70611)
1 µg peptide was spotted onto NC membrane and blotted using ab70608 (Lane 1) or ab70611 (Lane 2) at 1/1000 dilution. A:MEK5 (pS311/pT315), B: MEK5 (Non-phosphorylated), C: Non-related phosphospecific PP.
References for Anti-MEK5 antibody (ab70611)
has not yet been referenced specifically in any publications.
Publishing research using ab70611? Please let us know so that we can cite the reference in this datasheet.
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