Recombinant
RabMAb

Anti-MEK5 antibody [EP648Y] (ab45146)

Overview

  • Product name
    Anti-MEK5 antibody [EP648Y]
    See all MEK5 primary antibodies
  • Description
    Rabbit monoclonal [EP648Y] to MEK5
  • Specificity
    The immunogen used for this product shares 89% homology with MAP2K4/MEK4. Cross-reactivity with this protein has not been confirmed experimentally.
  • Tested applications
    Suitable for: WB, ICC/IF, Flow Cytmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to residues within human MEK5.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab45146 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ICC/IF
Flow Cyt
  • Application notes
    ICC/IF: 1/100 - 1/250.

    WB: 1/10000. Predicted molecular weight: 49 kDa.

    Is unsuitable for IHC, FACS or IP.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Acts as a scaffold for the formation of a ternary MAP3K2/MAP3K3-MAP3K5-MAPK7 signaling complex. Activation of this pathway appear to play a critical role in protecting cells from stress-induced apopotosis, neuronal survival and cardiac development and angiogenesis.
    • Tissue specificity
      Expressed in many adult tissues. Abundant in heart and skeletal muscle.
    • Sequence similarities
      Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
      Contains 1 OPR domain.
      Contains 1 protein kinase domain.
    • Domain
      Binds MAP3K2/MAP3K3 and MAPK7 via non-overlapping residues of the OPR domain. This domain also mediates interactions with SQSTM1 and PARD6A.
    • Post-translational
      modifications
      Activated by phosphorylation on Ser/Thr by MAP kinase kinase kinases.
      Yersinia yopJ may acetylate Ser/Thr residues, preventing phosphorylation and activation, thus blocking the MAPK signaling pathway.
    • Information by UniProt
    • Database links
    • Alternative names
      • Dual specificity mitogen activated protein kinase kinase 5 antibody
      • Dual specificity mitogen-activated protein kinase kinase 5 antibody
      • EC 2.7.12.2 antibody
      • HsT17454 antibody
      • MAP kinase kinase 5 antibody
      • MAP kinase kinase MEK5b antibody
      • MAP2K5 antibody
      • MAPK/ERK kinase 5 antibody
      • MAPKK 5 antibody
      • MAPKK5 antibody
      • MEK 5 antibody
      • mitogen-activated protein kinase kinase 5 antibody
      • MKK5 antibody
      • MP2K5_HUMAN antibody
      • PRKMK5 antibody
      • Protein kinase, mitogen-activated, kinase 5 antibody
      • SAPKK5 antibody
      • SKK5 antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence analysis of JAR (Human placenta choriocarcinoma epithelial cell) cells labeling MEK5 with Purified ab45146 at 1:250 dilution (8.1μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 μg/ml). ab150077 Goat anti rabbit IgG(Alexa Fluor® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

    • Anti-MEK5 antibody [EP648Y] (ab45146) at 1/1000 dilution (purified) + HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 20 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size : 49 kDa
      Observed band size : 49 kDa

      Blocking and diluting buffer: 5% NFDM/TBST

    • Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling MEK5 with purified ab45146 at 1:200 dilution (10 ug/ml) (red). Cells were fixed with 4% Paraformaldehyde and permeabilized with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

    • Immunofluorescent staining of HeLa using unpurified ab45146.

    • Anti-MEK5 antibody [EP648Y] (ab45146) at 1/10000 dilution (unpurified) + HeLa cell lysate

      Predicted band size : 49 kDa
      Observed band size : 49 kDa
    • Unpurified ab45146 at 1/100 staining HeLa cells by ICC/IF. The cells were paraformaldehyde fixed and blocked with goat serum prior to incubation with the antibody for 45 minutes. An Alexa Fluor® 488 conjugated goat polyclonal antibody was used as the secondary. This antibody detects MEK5 in HeLa cells as a cytoplasmic protein.

      See Abreview

    References

    ab45146 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Thank you for contacting us. I am sorry you are experiencing difficulty with this antibody. Typically when there are black dots on the blot, the antibody is binding with the blocking agent and it is necessary to filter the blocking agent. Was the same ...

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    Thank you for contacting us.

    I understand your concerns about the quality of the antibody. I can confirm, that we would expect the antibody to work well. However, if you experience any problems with this product please do not hesitate and come...

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    Thank you for your enquiry.

    I can confirm that ab45146 antibody is sold as tissue culture supernatant. Unpurified antibodies, such as those sold as whole antiserum, ascites or tissue culture supernatant will not have a concentration stated on ...

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    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HeLa, passage 78)
    Loading amount
    20 µg
    Specification
    HeLa, passage 78
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
    Username

    Abcam user community

    Verified customer

    Submitted Apr 13 2007

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa, passage 78)
    Specification
    HeLa, passage 78
    Fixative
    Paraformaldehyde
    Blocking step
    Serum as blocking agent for 40 minute(s) · Concentration: 3%
    Username

    Abcam user community

    Verified customer

    Submitted Apr 05 2007

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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